Muscarinic Acetylcholine Receptor M4 antibody - Carboxyterminal end (ab41170)

Product Name 

Muscarinic Acetylcholine Receptor M4 antibody - Carboxyterminal end

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Muscarinic Acetylcholine Receptor M4 - Carboxyterminal end

Immunogen 

peptide analogue of the C terminal of rat muscarinic M4 receptor covalently attached onto a carrier protein.

Reacts with 
(species key)

Rat

Specificity 

Cross reactivity for ab41170:

M4 Muscarinic Receptor (469-478): 100%
M4 Muscarinic Receptor: 70%

No cross reactivity was observed in:

M1 Muscarinic Receptor(451-460)
M1 Muscarinic Receptor
M2 Muscarinic Receptor(457-466)
M2 Muscarinic Receptor
M3 Muscarinic Receptor(580-589)
M3 Muscarinic Receptor
M5 Muscarinic Receptor(519-531)
M5 Muscarinic Receptor

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: 1/500.
WB: 1/400. Predicted molecular weight: 53 kDa.
This antibody is very sensitive to blocking agent. We recommend using whole goats serum for optimal results. Avoid any bovine products (BSA and milk).

With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Rat brain tissue, whole brain homogenate and cultured cells.

Cellular localization 

Cell Membrane

Research areas 

Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Acetylcholine
Neuroscience >> Neurotransmission >> Receptors / Channels >> GPCR >> Muscarinic Receptors

Relevance 

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Lyophilised
Reconstitute the lyophilized pooled antisera with 0.1 ml of 10 mg/ml BSA and 0.01% Sodium Azide in PBS.

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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