Overview

  • Product nameAnti-Mutarotase antibodySee all Mutarotase primary antibodies ...
  • Description
    Rabbit polyclonal to Mutarotase
  • Tested applicationsELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 35-84 (WGCTITALEV KDRQGRASDV VLGFAELEGY LQKQPYFGAV IGRVANRIAK) of Human Mutarotase (NP_620156).

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/62500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Anti-Mutarotase antibody images

  • Anti-Mutarotase antibody (ab83238) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-Mutarotase antibody (ab83238)

ab83238 has not yet been referenced specifically in any publications.

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