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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-MyD88 antibody
See all MyD88 products (9) ...
Description
Rabbit polyclonal to MyD88
Tested applications
IP, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 279-296 of Human MyD88.
Epitope
CTKSWFWTRLAKALSLP
Positive control
Jurkat whole cell lysate.
General notes
MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 16461741)
WB: 1/500 - 1/1000.Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
IHC-P: Use a concentration of 4 µg/ml
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain.
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ99836
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Myeloid differentiation primary response gene antibody
- MYD 88 antibody
- Myd88 antibody
see all
Database links
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Entrez Gene: 301059 Rat
- Omim: 602170 Human
- SwissProt: Q99836 Human
- SwissProt: P22366 Mouse
- SwissProt: Q3TIB3 Mouse
- SwissProt: Q3U6W5 Mouse
see all
Anti-MyD88 antibody images:
Western blot - MyD88 antibody (ab2064)
Anti-MyD88 antibody (ab2064) at 1/500 dilution + Jurkat Whole Cell Lysate
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab2064)
ICC/IF image of ab2064 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2064, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyD88 antibody (ab2064)
ab2064 (4µg/ml) staining MyD88 in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of the epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-MyD88 antibody (ab2064)
This product has been referenced in:
- Wang YLet al. Innate Immune Function of the Adherens Junction Protein p120-Catenin in Endothelial Response to Endotoxin. J Immunol 186:3180-7 (2011). WB; Mouse.Read more (PubMed: 21278343) »
- Steib CJet al. Intraperitoneal LPS amplifies portal hypertension in rat liver fibrosis. Lab Invest 90:1024-32 (2010). WB; Rat.Read more (PubMed: 20212458) »
See all 8 publications for this product
Publishing research using ab2064? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"