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ab2068 has been referenced in 5 publications.
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Anti-MyD88 antibody (ab2068) at 1 µg/ml + Jurkat whole cell lysate
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.
ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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