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thanks for your answer but I knew about peptide sequence I was asking if the 2 sequence are located in a very specific and interesting region ogf the protein?? I also was asking you about which is working best for western?? |
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ANSWER: |
All the information that we have regarding these two antibodies is located on the online datasheets. Both have been tested for application in Western blotting and shown to cross-react with human and mouse, but I really can't say which one works "best." |
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do you have data to send with any of them application: *on HEK 293 cell line and also on transfected cell with MyD88 gene *in sucrose fraction to detect protein raft which of the 2 polyclonal do work best and which specific region do they recognize
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ANSWER: |
Thank you very much for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets. Ab2064 corresponds to amino acids 279-296 of Human MyD88 (epitope is CTKSWFWTRLAKALSLP). Ab2068 corresponds to amino acids 233 to 248 of human MyD88 (5) (epitope is CDFQTKFALSLSPGAHD). If you have any more questions, please contact us again.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-MyD88 antibody (ab2068) at 1 µg/ml + Jurkat whole cell lysate
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.
ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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