Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-MyD88 antibody
See all MyD88 products (9) ...
Description
Rabbit polyclonal to MyD88
Tested applications
ICC/IF, IHC-P, WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Cow, Human
Immunogen
Peptide sequence corresponding to amino acids 233 to 248 of human MyD88, which differ from those of mouse by two amino acids.
Epitope
CDFQTKFALSLSPGAHD
Positive control
wB: Jurkat whole cell lysate IHC-P: human heart tissue
General notes
MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. My D88 gene is expressed in many tissues.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
ICC/IF
Flow Cyt
Application notes
ICC/IF: Use at a concentration of 5 µg/ml.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0, before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Use a modified RIPA lysis buffer: 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Remove cell debris by centrifugation. Boil the lysate for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol). Block the membrane with 5% non-fat dry milk in TBS overnight at 4C or 2 hours at room temperature. Detects a band of approximately 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user
Target
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain.
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ99836
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Myeloid differentiation primary response gene antibody
- MYD 88 antibody
- Myd88 antibody
see all
Database links
- Entrez Gene: 444881 Cow
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Omim: 602170 Human
- SwissProt: Q49BZ6 Cow
- SwissProt: Q599T9 Cow
- SwissProt: Q99836 Human
- SwissProt: P22366 Mouse
see all
Anti-MyD88 antibody images:
Western blot - MyD88 antibody (ab2068)
Anti-MyD88 antibody (ab2068) at 1 µg/ml + Jurkat whole cell lysate
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyD88 antibody (ab2068)
Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.
Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab2068)
ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MyD88 antibody (ab2068)
This product has been referenced in:
- Pakala SBet al. MTA1 coregulator regulates LPS response via MyD88-dependent signaling. J Biol Chem 285:32787-92 (2010). WB; Mouse.Read more (PubMed: 20702415) »
- Tomchuck SLet al. Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. Stem Cells 26:99-107 (2008). Flow Cyt; Human.Read more (PubMed: 17916800) »
See all 5 publications for this product
Publishing research using ab2068? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"