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Read our guarantee »Products:Signal Transduction >> Adapters >> Cytoplasmic
Anti-MyD88 antibody
See all MyD88 products (9) ...
Rabbit polyclonal to MyD88
ICC/IF, IHC-P, WB, Flow Cytmore details
Reacts with
Mouse, Cow, Human
Peptide sequence corresponding to amino acids 233 to 248 of human MyD88, which differ from those of mouse by two amino acids.
CDFQTKFALSLSPGAHD
wB: Jurkat whole cell lysate IHC-P: human heart tissue
Liquid
Store at +4°C.
PBS with 0.02% sodium azide
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
Our Abpromise guarantee covers the use of ab2068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 5 µg/ml.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0, before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Use a modified RIPA lysis buffer: 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Remove cell debris by centrifugation. Boil the lysate for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol). Block the membrane with 5% non-fat dry milk in TBS overnight at 4C or 2 hours at room temperature. Detects a band of approximately 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Ubiquitous.
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Contains 1 death domain.
Contains 1 TIR domain.
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
Cytoplasm.
Target information above from: UniProt accessionQ99836
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MyD88 antibody (ab2068)

Anti-MyD88 antibody (ab2068) at 1 µg/ml + Jurkat whole cell lysate
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyD88 antibody (ab2068)

Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.
Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab2068)

ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
See all 5 publications for this product
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Anti-MyD88 antibody (ab2068) at 1 µg/ml + Jurkat whole cell lysate
Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.

ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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