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Western blot - MyD88 antibody (ab2068)
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Immunohistochemistry (Paraffin-embedded sections) - MyD88 antibody (ab2068)
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Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab2068)
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Product Name
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MyD88 antibody
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See all MyD88 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MyD88
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Immunogen
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Peptide sequence corresponding to amino acids 233 to 248 of human MyD88, which differ from those of mouse by two amino acids.
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Reacts with
(species key)
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Hu, Ms, Cow
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Epitope
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CDFQTKFALSLSPGAHD
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
Flow Cyt: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0, before commencing with IHC staining protocol.
WB: 1/500 - 1/1000. Use a modified RIPA lysis buffer: 150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Remove cell debris by centrifugation. Boil the lysate for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol). Block the membrane with 5% non-fat dry milk in TBS overnight at 4C or 2 hours at room temperature. Detects a band of approximately 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user
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Positive control
(see definition)
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wB: Jurkat whole cell lysate
IHC-P: human heart tissue
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL1R and is involved in the inflammatory response induced by IL1, IL18 and LPS. MyD88 associates with and recruits IRAK to IL1 receptor complex in response to IL1. This pathway further leads to activation of NFkB. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL1 and IL18, and MyD88 deficient mice lack responses to bacterial product LPS that employs Toll like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 has a wide tissue distribution.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.02% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. My D88 gene is expressed in many tissues.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MyD88 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MyD88 antibody images, references, products related to ab2068 and other tools.
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MyD88 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - MyD88 antibody (ab2068)
Immunohistochemical staining (paraffin embedded) of human heart tissue using anti-MyD88 at 2 µg/ml.
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Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab2068)
ICC/IF image of ab2068 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2068, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for MyD88 antibody (ab2068)
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This product has been used in: (two most recent references)
Tomchuck SL et al. Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses. Stem Cells 26:99-107 (2008). Flow Cyt; Human. PubMed: 17916800
Delgado MA et al. Toll-like receptors control autophagy. EMBO J : (2008). WB; Mouse. PubMed: 18337753
See all 4 publication references for this product.
If you publish research using ab2068 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MyD88
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MyD88 antibody - more information
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MyD88 antibody - related products:
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MyD88 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MyD88 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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