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Western blot - MyD88 antibody (ab28763)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyD88 antibody (ab28763)
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Western blot - MyD88 antibody (ab28763)
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Product Name
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MyD88 antibody
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See all MyD88 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to MyD88
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Immunogen
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Synthetic peptide: C-IKYKAM KKEFP, corresponding to Internal sequence amino acids 255-265 of Human MyD88.
BLAST 'C-IKYKAM KKEFP' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cow, Shp
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/64,000.
IHC-P: Use at a concentration of 2 - 5 µg/ml.
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human T cell line MOLT4 cell lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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Myeloid differentiation Marker 88 (MyD88) is an adaptor molecule for the Toll/IL1R and is involved in the inflammatory response induced by IL1, IL18 and LPS. MyD88 associates with and recruits IRAK to IL1 receptor complex in response to IL1. This pathway further leads to activation of NFkB. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL1 and IL18, and MyD88 deficient mice lack responses to bacterial product LPS that employs Toll like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 has a wide tissue distribution.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MyD88 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MyD88 antibody images, references, products related to ab28763 and other tools.
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MyD88 antibody images:
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 Western blot - MyD88 antibody (ab28763)
MyD88 antibody (ab28763) at 0.01 µg/ml + Human T cell line MOLT4 cell lysate, 30µg total protein per lane
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Primary incubation was for 1 hour.
Detected using chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyD88 antibody (ab28763)
ab28763 at 3µg/ml staining of MyD88 in human tonsil tissue section by Immunhistochemistry (Formalin/ PFA fixed paraffin embedded sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 6). The HRP-staining procedure was used for staining.
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Western blot - MyD88 antibody (ab28763)
MyD88 antibody (ab28763) at 0.03 µg/ml + Lysates prepared from human thymus at 35 µg/ml
developed using the ECL technique
Predicted band size : 33 kDa
Primary incubated for 1 hour.
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References for MyD88 antibody (ab28763)
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This product has been used in:
Szajnik M et al. TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Oncogene 28:4353-63 (2009). IHC-P; Human. PubMed: 19826413
If you publish research using ab28763 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MyD88
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MyD88 antibody - more information
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Customer reviews (feedback) regarding MyD88 antibody |
Customer FAQs regarding MyD88 antibody |
Protocols for MyD88 antibody |
Price and availability of products related to MyD88 antibody |
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MyD88 antibody - related products:
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MyD88 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MyD88 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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