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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-MyD88 antibody
See all MyD88 products (9) ...
Description
Goat polyclonal to MyD88
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Sheep, Cow, Dog
Immunogen
Synthetic peptide: C-IKYKAM KKEFP, corresponding to Internal sequence amino acids 255-265 of Human MyD88.
C-IKYKAM KKEFP
Positive control
Human T cell line MOLT4 cell lysate. Human thymus lysate. Human tonsil tissue sections.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 - 5 µg/ml.
WB: Use a concentration of 0.03 - 0.1 µg/ml.Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Target
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain.
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ99836
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Myeloid differentiation primary response gene antibody
- MYD 88 antibody
- Myd88 antibody
see all
Database links
- Entrez Gene: 444881 Cow
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Entrez Gene: 301059 Rat
- Omim: 602170 Human
- SwissProt: Q49BZ6 Cow
- SwissProt: Q599T9 Cow
- SwissProt: Q99836 Human
see all
Anti-MyD88 antibody images:
Western blot - MyD88 antibody (ab28763)
Anti-MyD88 antibody (ab28763) at 0.01 µg/ml + Human T cell line MOLT4 cell lysate, 30µg total protein per lane
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Primary incubation was for 1 hour.
Detected using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyD88 antibody (ab28763)
ab28763 at 3µg/ml staining of MyD88 in human tonsil tissue section by Immunhistochemistry (Formalin/ PFA fixed paraffin embedded sections). Tissue underwent antigen retrieval in microwave with citrate buffer (pH 6). The HRP-staining procedure was used for staining.
Western blot - MyD88 antibody (ab28763)
Anti-MyD88 antibody (ab28763) at 0.03 µg/ml + Lysates prepared from human thymus at 35 µg/ml
developed using the ECL technique
Predicted band size : 33 kDa
Primary incubated for 1 hour.
References for Anti-MyD88 antibody (ab28763)
This product has been referenced in:
- Szajnik Met al. TLR4 signaling induced by lipopolysaccharide or paclitaxel regulates tumor survival and chemoresistance in ovarian cancer. Oncogene 28:4353-63 (2009). IHC-P; Human.Read more (PubMed: 19826413) »
See 1 publication for this product
Publishing research using ab28763? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"