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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-MyD88 antibody
See all MyD88 products (9) ...
Description
Rabbit polyclonal to MyD88
Tested applications
WB, ICC/IF, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human MyD88.
(Peptide available as ab49768.)
Positive control
This antibody gave a positive signal in Jurkat Whole Cell Lysate and mouse lung tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 35 kDa (predicted molecular weight: 33 kDa).
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: Use at an assay dependent dilution.
Target
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
Tissue specificity
Ubiquitous.
Involvement in disease
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
Sequence similarities
Contains 1 death domain.
Contains 1 TIR domain.
Domain
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ99836
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Myeloid differentiation primary response gene antibody
- MYD 88 antibody
- Myd88 antibody
see all
Database links
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Entrez Gene: 396646 Pig
- Entrez Gene: 301059 Rat
- Omim: 602170 Human
- SwissProt: Q99836 Human
- SwissProt: P22366 Mouse
- SwissProt: Q3TIB3 Mouse
see all
Anti-MyD88 antibody images:
Western blot - MyD88 antibody (ab36076)
Anti-MyD88 antibody (ab36076) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab36076)
ICC/IF image of ab36076 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36076, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-MyD88 antibody (ab36076)
Anti-MyD88 antibody (ab36076) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 33 kDa
Additional bands at : 65 kDa,70 kDa,85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
References for Anti-MyD88 antibody (ab36076)
ab36076 has not yet been referenced specifically in any publications.
Publishing research using ab36076? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"