Anti-MyD88 antibody (ab36076)

Overview

• Product nameAnti-MyD88 antibodySee all MyD88 primary antibodies ...
• Description
  Rabbit polyclonal to MyD88
• Tested applicationsWB, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Pig
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human MyD88.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab49768.)

• Positive controlThis antibody gave a positive signal in Jurkat Whole Cell Lysate and mouse lung tissue lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Adapters
  • Cytoplasmic

  • Cardiovascular
  • Atherosclerosis
  • Vascular Inflammation
  • Inflammatory mediators

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionAdapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway.
• Tissue specificityUbiquitous.
• Involvement in diseaseDefects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age.
• Sequence similaritiesContains 1 death domain.
  Contains 1 TIR domain.
• DomainThe intermediate domain (ID) is required for the phosphorylation and activation of IRAK.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q99836 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4615 Human
  • Entrez Gene: 17874 Mouse
  • Entrez Gene: 396646 Pig
  • Entrez Gene: 301059 Rat
  • Omim: 602170 Human
  • SwissProt: Q99836 Human
  • SwissProt: P22366 Mouse
  • SwissProt: Q6Y1S1 Rat

  • Unigene: 82116 Human
  • Unigene: 213003 Mouse
  • Unigene: 37341 Rat

  see all

• Alternative names
  MYD 88 antibodyMyd88 antibodyMYD88_HUMAN antibody

  MYD88D antibodyMyeloid differentiation marker 88 antibodyMyeloid differentiation primary response gene 88 antibodyMyeloid differentiation primary response gene antibodyMyeloid differentiation primary response protein MyD88 antibodyOTTHUMP00000161718 antibodyOTTHUMP00000208595 antibodyOTTHUMP00000209058 antibodyOTTHUMP00000209059 antibodyOTTHUMP00000209060 antibody

  see all

Anti-MyD88 antibody images

• 

  Western blot - MyD88 antibody (ab36076)
  Anti-MyD88 antibody (ab36076) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 33 kDa
  Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - MyD88 antibody (ab36076)
  ICC/IF image of ab36076 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36076, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Western blot - Anti-MyD88 antibody (ab36076)
  Anti-MyD88 antibody (ab36076) at 1 µg/ml + Lung (Mouse) Tissue Lysate at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 33 kDa
  Observed band size : 33 kDa
  Additional bands at : 65 kDa,70 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes