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Anti-Myc tag antibody (Agarose) (ab1253)

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Overview

Product name

Anti-Myc tag antibody (Agarose)
See all Myc tag products (16) ...

Description

Goat polyclonal to Myc tag (Agarose)

Conjugation

Agarose

Conjugation notes

Goat anti-c-myc affinity-purified antibodies were coupled to agarose beads using a cyanogen bromide method.

Tested applications

IPmore details

Immunogen

Full length native protein EQKLISEEDL (c-myc) (purified): conjugated to KLH.

EQKLISEEDL

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.1M Sodium chloride, 0.01M PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Applications

Show applications key

Our Abpromise guarantee covers the use of ab1253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IP: Use a concentration of 20 - 40 µg/ml.(Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.)

Application notes

Is unsuitable for or ChIP.

Target

Relevance

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Alternative names

  • c Myc antibody
  • c Myc Epitope Tag antibody
  • c Myc Tag antibody
  • Myc Epitope Tag antibody
  • Myc proto-oncogene protein antibody
  • Transcription factor p64 antibody
see all

References for Anti-Myc tag antibody (Agarose) (ab1253)

This product has been referenced in:

  • Epting Det al. The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish. Circ Res 107:45-55 (2010). IP.Read more (PubMed: 20466982) »
  • Thoma Cet al. Enhancement of IRES-mediated translation of the c-myc and BiP mRNAs by the poly(A) tail is independent of intact eIF4G and PABP. Mol Cell 15:925-35 (2004).Read more (PubMed: 15383282) »

See all 2 publications for this product

Publishing research using ab1253? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"