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ab13837 |
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ab51501 |
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ab52579 |
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ab46666 |
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ab37373 |
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Read our guarantee »Publishing research using ab9132? Please let us know so that we can cite the reference in this datasheet
ab9132 has been referenced in 9 publications.
Publishing research using ab9132? Please let us know so that we can cite the reference in this datasheet
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A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23ºC. The primary antibody incubated with the sample for 1 hour at 23ºC. A donkey polyclonal anti-mouse antibody, diluted 1/500, was used as the secondary.
This image is courtesy of an anonymous Abreview.
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