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ab13837 |
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ab51501 |
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ab52579 |
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ab46666 |
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ab37373 |
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Will this antibody detect endogenous c-myc in mouse? |
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ANSWER: |
Thank you for your enquiry. Unfortunately the antibody has not been tested on endogenous mouse protein, so there is no data to say either way. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further. |
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Do you know if this antibody works for immunofluorescence? |
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ANSWER: |
No, this antibody has not yet been tested in IF |
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Will this react with human N-myc as well? |
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ANSWER: |
Cross reactivity to n-myc has not been tested. The immunogen was the peptide sequence EQKLISEEDL. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23ºC. The primary antibody incubated with the sample for 1 hour at 23ºC. A donkey polyclonal anti-mouse antibody, diluted 1/500, was used as the secondary.
This image is courtesy of an anonymous Abreview.
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