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ChIP - Myc tag antibody - ChIP Grade (ab9132)
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Product Name
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Myc tag antibody - ChIP Grade
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See all Myc tag antibodies (14)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Myc tag - ChIP Grade
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Immunogen
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Synthetic peptide: EQKLISEEDL (c-myc) conjugated to KLH.
BLAST 'EQKLISEEDL' with  or 
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Specificity
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Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).
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Tested applications
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ChIP, ELISA, ICC/IF, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads
ELISA: 1/100 - 1/500.
ICC: 1/200 - 1/2000.
IHC-Fr: 1/50.
WB: 1/1000 - 1/30000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Tags & Cell Markers >> Epitope Tags >> Myc Tag
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Relevance
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Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS with 0.1% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were affinity purified using the peptide immobilized on solid support.
Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Myc tag antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Myc tag antibody - ChIP Grade images, references, products related to ab9132 and other tools.
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Myc tag antibody - ChIP Grade images:
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 ChIP - Myc tag antibody - ChIP Grade (ab9132)
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
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References for Myc tag antibody - ChIP Grade (ab9132)
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This product has been used in: (two most recent references)
Aguilar-Arnal L et al. The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching. EMBO J 27:1029-38 (2008). ChIP. PubMed: 18354497
Beierle EA et al. N-MYC regulates focal adhesion kinase expression in human neuroblastoma. J Biol Chem 282:12503-16 (2007). PubMed: 17327229
If you publish research using ab9132 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Myc tag
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