Anti-Myc tag antibody - ChIP Grade (ab9132)

  ChIP - Myc tag antibody - ChIP Grade (ab9132)

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

  Immunocytochemistry/ Immunofluorescence - Myc tag antibody - ChIP Grade (ab9132)

ab9132, at 1/500, tagging the Myc epitope in human Hela cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in 0.15% Triton prior to blocking in 10% serum for 1 hour at 23°C. The primary antibody incubated with the sample for 1 hour at 23°C. A donkey polyclonal anti-mouse antibody, diluted 1/500, was used as the secondary.

This image is courtesy of an anonymous Abreview.

See Abreview