Anti-Myc tag antibody [Myc.A7] (ab18185)

Hello there
I have purchased monoclonal Myc Ab.
http://www.abcam.com/Myc-tag-antibody-Myc-A7-ab18185.html
I would like to know what is the Kd for this antibody against the Myc
epitope.It would be quite helpful for me.
Regards

ANSWER:

Thank you for your enquiry,

I am sorry to confirm that the Kd for ab18185 Myc tag antibody [Myc.A7] has not been assessed. It is unusual for the Kd to be measured for research grade antibodies.

We aim to provide as much information as possible to customers, and I am sorry we do not have the details you require on this occasion. If you have any further questions, please do not hesitate to contact us.

Heu there
Thanks for the reply
We have selected some peptide aptamers against Myc antibodies.we have
the myc epitopes in the loops of the peptide aptamers.the selection
was done using polyclonal antibodies.It was an ELISA detection.Now we
would like to shift to a monoclonal Ab because we think that using
monoclonal Ab would be more specific and more appropriate for
comparing the dissociation constants of the different binders.
Regards

ANSWER:

Thank you for getting back to me and for providing some further information about your research project.

Currently, we have 2 monoclonal antibodies against Myc tag: ab18185 and ab94936. Although they are from the same clone but from two sources and the testing conditions were different. ab94936 reacts with recombinant fragment so the other antibody ab18185 may suit your need better.

There are two specific publication for ab18185 you could take a look at to get some more information:

Biswas Ket al. A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay. Blood 118:2430-42 (2011). WB.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21719596&dopt=Abstract
Ohe Met al. Emi2 inhibition of the anaphase-promoting complex/cyclosome absolutely requires Emi2 binding via the C-terminal RL tail. Mol Biol Cell 21:905-13 (2010). WB.http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20089832&dopt=Abstract



If you need any further assistance in the future, please do not hesitate to contact me.