Myc tag antibody [Myc.A7] (ab18185)

Product Name 

Myc tag antibody [Myc.A7]

Product type 

Primary antibodies

Description 

Mouse monoclonal [Myc.A7] to Myc tag

Immunogen 

Synthetic peptide: EQKLISEEDL, corresponding to amino acids 410-419 of Human Myc.
BLAST 'EQKLISEEDL' with or

Reacts with 
(species key)

N/A

Specificity 

Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.

Tested applications 
(see key)

Dot, ELISA, ICC/IF, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions

Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC: 1/200.
IF: 1/200.
IP: Use at an assay dependent dilution.
WB: 1/1000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Tags & Cell Markers >> Epitope Tags >> Myc Tag

Relevance 

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

Myc.A7

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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