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Read our guarantee »Products:Tags & Cell Markers >> Epitope Tags >> Myc Tag
Anti-Myc tag antibody [Myc.A7]
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Mouse monoclonal [Myc.A7] to Myc tag
Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
ICC/IF, ELISA, Dot Blot, WB, IP, ICC, IFmore details
Synthetic peptide: EQKLISEEDL, corresponding to amino acids 410-419 of Human Myc.
EQKLISEEDL
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
IgG fraction
Monoclonal
Myc.A7
IgG1
Immunocytochemistry/ Immunofluorescence - Myc tag antibody [Myc.A7] (ab18185)
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Our Abpromise guarantee covers the use of ab18185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
ELISA: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
WB: 1/1000
IP: Use at an assay dependent dilution.
ICC: 1/200
IF: 1/200
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Immunocytochemistry/ Immunofluorescence - Myc tag antibody [Myc.A7] (ab18185)
![Immunocytochemistry/ Immunofluorescence - Myc tag antibody [Myc.A7] (ab18185)](/ps/datasheet/images/18/ab18185/Myc-tag-Primary-antibodies-ab18185-3.jpg)
Top Panel: ab18185 staining Myc Tag in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-3% BSA) for 30 minutes at 20°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (1/500) was used as secondary antibody.
Bottom panel: Synapses (Red); Anti-myc tag (Green)
This image is courtesy of an anonymous Abreview
This product has been referenced in:
See all 2 publications for this product
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![Immunocytochemistry/ Immunofluorescence - Myc tag antibody [Myc.A7] (ab18185)](/ps/datasheet/images/18/ab18185/Myc-tag-Primary-antibodies-ab18185-3.jpg)
Top Panel: ab18185 staining Myc Tag in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-3% BSA) for 30 minutes at 20°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG (1/500) was used as secondary antibody.
Bottom panel: Synapses (Red); Anti-myc tag (Green)
This image is courtesy of an anonymous Abreview
1
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