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Products:Tags & Cell Markers >> Epitope Tags >> Myc Tag
Overview
Product name
Anti-Myc tag antibody
See all Myc tag products (16) ...
Description
Rabbit polyclonal to Myc tag
Specificity
ELISA: Antibody specificity was verified by ELISA against the peptide (EQKLISEEDL). A 1:60,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP-conjugated Goat Anti Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.
Tested applications
IHC-Fr, IP, WB, IHC-P, Flow Cyt, ICC/IF, ICC, Electron Microscopymore details
Immunogen
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase. Antibody concentration was determined by extinction coefficient : O.D. 1.4 at 280nM equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/500
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution. (PubMed: 19793804)
ICC/IF: Use at an assay dependent dilution. (PubMed: 16093346)
ICC: Use at an assay dependent concentration.
EM: Use at an assay dependent dilution. (See Abreview for further details (submitted by Eeva-Liisa Eskelinen).)
Target
Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Alternative names
- c Myc antibody
- c Myc Epitope Tag antibody
- c Myc Tag antibody
see all
Anti-Myc tag antibody images:
Immunoprecipitation - Myc tag antibody (ab9106)
Ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate). In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.
Lane 1: Input untransfected cells
Lane 2: Immunoprecipitated untransfected cells
Lane 3: Input co-transfected cells
Lane 4: Immunoprecipitated co-transfected cells
This image is courtesy of an Abreview submitted on 23 March 2006.
Electron Microscopy - Myc tag antibody (ab9106)
ab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC. The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10nm Gold conjugated) was used as the secondary. The image shown is immuno electron microscopical staining with thawed cryosections.
This image is courtesy of an Abreview submitted by Dr Eeva-Liisa Eskelinen
Immunoprecipitation - Myc tag antibody (ab9106)
ab9106 at 4µg/mg lysate used in human huh-7 whole cell lysate (1x106 cells). Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution ab17437.
This image is courtesy of an anonymous abreview.
Immunocytochemistry/ Immunofluorescence - Myc tag antibody (ab9106)
ab9106 staining the Myc tag in HeLa cells transfected with a Myc-tagged membrane targeting protein by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X (0.5%) and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/500) for 1 hour at 24°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-Myc tag antibody (ab9106)
This product has been referenced in:
- Leonhardt RMet al. Proprotein convertases process Pmel17 during secretion. J Biol Chem : (2011). WB.Read more (PubMed: 21247888) »
- Muñoz-Descalzo Set al. Modulation of the ligand-independent traffic of Notch by Axin and Apc contributes to the activation of Armadillo in Drosophila. Development 138:1501-6 (2011).Read more (PubMed: 21389052) »
See all 61 publications for this product
Publishing research using ab9106? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"