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Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] (ab51804)

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Overview

Product name

Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46]

Description

Mouse monoclonal [WHO no. IT-46] to Mycobacterium L-alanine dehydrogenase

Specificity

ab51804 reacts specifically with Ald of Mycobacterium tuberculosis, Mycobacterium marinum but not Mycobacterium bovis BCG. Does not react with pyridine nucleotide transhydrogenase (PNT) of Mycobacterium tuberculosis.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with Mycobacterium tuberculosis and M. marinum. Does not react with M. bovis BCG. Not yet tested in other species.

Immunogen

Culture filtrate from Mycobacterium tuberculosis H37Rv

Epitope

Recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

WHO no. IT-46

Isotype

IgG1

Light chain type

kappa

Research areas

Signal Transduction >> Metabolism >> Amino Acids
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Mycobacterium

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Mycobacterium L-alanine dehydrogenase (ald) belongs to the AlaDH/PNT family and is a 40 kDa secreted protein from short term culture of Mycobacterium tuberculosis; which may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.

Cellular localization

Secreted

Alternative names

  • 40 kDa antigen antibody
  • Alanine dehydrogenase antibody
  • ald antibody
  • TB43 antibody

References for Anti-Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] (ab51804)

This product has been referenced in:

  • Hutter B & Singh M Host vector system for high-level expression and purification of recombinant, enzymatically active alanine dehydrogenase of Mycobacterium tuberculosis. Gene 212:21-9 (1998).Read more (PubMed: 9661660) »

See 1 publication for this product

Publishing research using ab51804? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"