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Product Name
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Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46]
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [WHO no. IT-46] to Mycobacterium L-alanine dehydrogenase
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Immunogen
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Culture filtrate from Mycobacterium tuberculosis H37Rv
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Reacts with
(species key)
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N/A
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Specificity
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ab51804 reacts specifically with Ald of Mycobacterium tuberculosis, Mycobacterium marinum but not Mycobacterium bovis BCG. Does not react with pyridine nucleotide transhydrogenase (PNT) of Mycobacterium tuberculosis.
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Epitope
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Recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of Mycobacterium tuberculosis.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 40 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Signal Transduction >> Metabolism >> Amino Acids
Microbiology >> Organism >> Bacteria >> Gram positive bacteria >> Mycobacterium
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Relevance
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Mycobacterium L-alanine dehydrogenase (ald) belongs to the AlaDH/PNT family and is a 40 kDa secreted protein from short term culture of Mycobacterium tuberculosis; which may play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.
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Alternative names Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] (ab51804) |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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WHO no. IT-46
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] (ab51804)
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This product has been used in:
Hutter B & Singh M Host vector system for high-level expression and purification of recombinant, enzymatically active alanine dehydrogenase of Mycobacterium tuberculosis. Gene 212:21-9 (1998). PubMed: 9661660
If you publish research using ab51804 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Mycobacterium L-alanine dehydrogenase
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Mycobacterium L-alanine dehydrogenase antibody [WHO no. IT-46] - more information
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