Myelin Basic Protein (Tagged) (ab43614)
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
Our Abpromise guarantee covers the use of ab43614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||FuncS: Use at an assay dependent dilution.|
- GDBGolli MBPGolli MBP; myelin basic protein
- Hemopoietic MBPHMBPRHUGOMBPmbpMBP_HUMANMGC99675MLDMyelin A1 proteinMyelin basic proteinMyelin DeficientMyelin membrane encephalitogenic proteinOTTHUMP00000163776OTTHUMP00000174387OTTHUMP00000174388SHIShivererSP
modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
References for Myelin Basic Protein (Tagged) (ab43614)
ab43614 has not yet been referenced specifically in any publications.