Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)

Do you know the concentration of this antibody, or the IgG range?

ANSWER:

Thank you for contacting Abcam.
I have heard back from the lab already and they have told me that we do not measure the concentration of our tissue culture supernatants because of the other proteins in the supernatant that would interfere with the reading. Generally in these preparations, we expect 10-50ug/ml specific antibody.
Please let me know if you have any additional questions.

We frequently use your MBP antibody (ab7349) and we were wondering whether we can purchase this antibody already conjugated to a fluorophore?If this is possible please provide me a quote and estimate of how long it would take.

We typically bindab7349withAlexa Fluor 488 secondary. We would consider other fluorophores.

ANSWER:

Thank you for your patience.

I have heard back from the supplying lab.

Yes, it is certainly a possibility that we could provide this purified on a custom basis.Due to licensing restrictions on the Alexa and Dylight dyes,we would not be able to offer any of those. This leaves the standards RPE, FITC, APC, etc. The closest to Alexa488 would be FITC.

Alternatively, we could provide the purified material, andyoucould conjugate the material as described in the previous email (using the EasyLink kits - http://www.abcam.com/EasyLink).

I hope this information is helpful to you. if you have further questions regarding custom or bulk orders, please send them directly to sales@abcam.com.

Thank you for your continued business.

We frequently use your MBP antibody (ab7349) and we were wondering whether we can purchase this antibody already conjugated to a fluorophore?If this is possible please provide me a quote and estimate of how long it would take.

We typically bindab7349withAlexa Fluor 488 secondary. We would consider other fluorophores.

ANSWER:

Thank you for contacting us.

I have contacted the supplying lab to check if a custom conjugation might be possible. For custom orders we usually require an order of at least 10 vials.

Alternatively, you could conjugate the antibody yourself, using our EasyLink kits:

http://www.abcam.com/EasyLink

We do not offer AlexaFluor fluorophores, but have various other conjugates as well as different sizes from 3x10ug to 1x1mg. Please check the link above for the full list of conjugation kits that we have.

I will let you know what I hear back from the lab.

I hope this information is of some help to you. Please do not hesitate to contact us if you need any more advice or information.

I would like to test 4 antibodies in IHC-P canine tissue. The ab cat. nos are ab68779 (ChAT), ab14184 (Sub. P), ab65832 (Nic. Ac. R. Alpha 1), and ab7349 (MBP). Thanks

ANSWER:

DISCOUNT CODES:
ab68779 - **********
ab14184 - ***********
ab65832 - ******************
ab7349 - ***************

Expiration date fro all discount codes : *********

I am pleased to hear you would like to accept our offer and test abab68779 (ChAT), ab14184 (Sub. P), ab65832 (Nic. Ac. R. Alpha 1), and ab7349 (MBP) in canine tissue.. This code will give you 1 free PRIMARY ANTIBODY before the expiration date for each antibody tested. To redeem this offer, please submit an Abreview for canine tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

I would appreciate it if you could let me know the accession # of the specific bovine immunogen that has been used to raise the antibody ab7349. Also if you could tell me what the amino acid sequence 82-87 is to which the ab binds, that would be great.

ANSWER:

Thank you for your enquiry.

I do not have the accession number for the immunogen; however, the amino acid sequence to which the antibody binds is DENPVV. I have updated the datasheet for future reference.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.