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Immunohistochemistry (Frozen sections) - Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)
![Immunohistochemistry (Frozen sections) - Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)](/ps/datasheet/images/ab7349_2.jpg)
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Product Name
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Myelin Basic Protein antibody [12] - Oligodendrocyte Marker
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See all Myelin Basic Protein antibodies (23)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [12] to Myelin Basic Protein - Oligodendrocyte Marker
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Immunogen
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Full length protein (Cow).
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Gpig, Pig, Rb, Shp
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Specificity
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The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Mapped by Geyson method to DENPW method.
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Epitope
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Amino acids 82-87 (DENPVV).
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Tested applications
(see key)
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ELISA, IHC (PFA fixed), IHC-FoFr, IHC-Fr, IHC-P, RIA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
Immunohistochemistry (PFA fixed): 1/100 (from reference PubMed: 17065439).
IHC-FoFr: Use at an assay dependent dilution (PMID 18678887).
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic side of myelin.
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins
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Relevance
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The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
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Alternative names Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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12
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Myeloma
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NS0
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Isotype
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IgG2a
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: Tissue Culture Supernatant Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Myelin Basic Protein antibody [12] - Oligodendrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Myelin Basic Protein antibody [12] - Oligodendrocyte Marker images, references, products related to ab7349 and other tools.
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Myelin Basic Protein antibody [12] - Oligodendrocyte Marker images:
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 Immunohistochemistry (Frozen sections) - Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)
ab7349 at 1/100 staining mouse spinal cord tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 3% serum prior to incubation with the antibody for 18 hours. A biotinylated horse anti-rat antibody was used as the secondary. This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy
See Abreview
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References for Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)
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This product has been used in: (two most recent references)
Fujino H et al. Axonal protection by brain-derived neurotrophic factor associated with CREB phosphorylation in tumor necrosis factor-alpha-induced optic nerve degeneration. Acta Neuropathol 117:75-84 (2009). IHC-P; Rat. PubMed: 18830614
Yu WM et al. Laminin is required for Schwann cell morphogenesis. J Cell Sci 122:929-36 (2009). ICC/IF; Mouse. PubMed: 19295124
See all 12 publication references for this product.
If you publish research using ab7349 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Myelin Basic Protein
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Myelin Basic Protein antibody [12] - Oligodendrocyte Marker - more information
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Protocols for Myelin Basic Protein antibody [12] - Oligodendrocyte Marker |
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Myelin Basic Protein antibody [12] - Oligodendrocyte Marker - related products:
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Myelin Basic Protein antibody [12] - Oligodendrocyte Marker - other tools:
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Contact Abcam with a Technical Enquiry about Myelin Basic Protein antibody [12] - Oligodendrocyte Marker |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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