Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)

Overview

• Product nameAnti-Myelin Basic Protein antibody [12] - Oligodendrocyte MarkerSee all Myelin Basic Protein primary antibodies ...
• Description
  Rat monoclonal [12] to Myelin Basic Protein - Oligodendrocyte Marker
• SpecificityThe antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Mapped by Geyson method to DENPW method.
• Tested applicationsIHC-FoFr, IHC-P, WB, ELISA, RIA, IHC-Fr more details
• Species reactivity
  Reacts with: Mouse, Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Human, Pig, Apteronotus leptorhynchus
  
• Immunogen

  Full length protein (Cow).

• EpitopeAmino acids 82-87 (DENPVV).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: Tissue Culture Supernatant
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone number12
• MyelomaNS0
• IsotypeIgG2a
• Research Areas
  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Adhesion Proteins
  • Membrane Proteins

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Oligodendrocyte marker

Applications

Target

• FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
• Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
• Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
• Sequence similaritiesBelongs to the myelin basic protein family.
• Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
• Post-translational
  modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
  The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
  Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
• Cellular localizationMyelin membrane. Cytoplasmic side of myelin.

Target information above from: UniProt accession P02686 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 618684 Cow
  • Entrez Gene: 476160 Dog
  • Entrez Gene: 4155 Human
  • Entrez Gene: 17196 Mouse
  • Entrez Gene: 414286 Pig
  • Entrez Gene: 24547 Rat
  • Omim: 159430 Human
  • SwissProt: P02687 Cow

  • SwissProt: P25188 Guinea pig
  • SwissProt: P02686 Human
  • SwissProt: P04370 Mouse
  • SwissProt: P81558 Pig
  • SwissProt: P25274 Rabbit
  • SwissProt: P02688 Rat
  • Unigene: 551713 Human
  • Unigene: 252063 Mouse
  • Unigene: 392350 Mouse
  • Unigene: 454459 Mouse
  • Unigene: 63285 Rat

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• Alternative names
  GDB antibodyGolli MBP antibodyGolli MBP; myelin basic protein antibody

  Hemopoietic MBP antibodyHMBPR antibodyHUGO antibodyMBP antibodyMBP_HUMAN antibodyMGC99675 antibodyMLD antibodyMyelin A1 Protein antibodyMyelin basic protein antibodyMyelin Deficient antibodyMyelin Membrane Encephalitogenic Protein antibodyOTTHUMP00000163776 antibodyOTTHUMP00000174387 antibodyOTTHUMP00000174388 antibodySHI antibodyShiverer antibodySP antibody

  see all

Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker images

• 

  Immunohistochemistry (Frozen sections) - Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy
  ab7349 at 1/100 staining mouse spinal cord tissue sections by IHC-Fr. The tissue was formaldehyde fixed and blocked with 3% serum prior to incubation with the antibody for 18 hours. A biotinylated horse anti-rat antibody was used as the secondary.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)Image courtesy of an Abreview submitted by Guillermo Estivill-Torrus.
  ab7349 staining Myelin Basic Protein in murine cerebral cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was not fixed. Samples were blocked with 2.5% serum for 1 hour followed by incubation with the primary antibody at a 1/200 dilution for 18 hours. A Biotin-conjugated rabbit anti-rat polyclonal was used as the secondary antibody at a 1/500 dilution.
• 

  Western blot - Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349)
  Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker (ab7349) + Mouse brain tissue lysate
  
  Observed band size : 19,26 kDa (why is the actual band size different from the predicted?)