Anti-Myelin Basic Protein antibody [7D2] (ab78157)

Overview

• Product nameAnti-Myelin Basic Protein antibody [7D2]See all Myelin Basic Protein primary antibodies ...
• Description
  Mouse monoclonal [7D2] to Myelin Basic Protein
• Specificityab78156 detects four transcripts (molecular weights of 21.5, 18.5, 17.2, 14 kDa) with much stronger binding for the top two bands.
• Tested applicationsWB, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Purified bovine myelin.

• Positive controlCrude rat spinal cord homogenate. Rat peripheral nerve homogenate. Rat mixed neuron/glial cultures.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number7D2
• IsotypeIgG1
• Research Areas
  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Oligodendrocyte marker

  • Neuroscience
  • Cell Adhesion Proteins
  • Membrane Proteins

Applications

Target

• FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
• Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
• Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
• Sequence similaritiesBelongs to the myelin basic protein family.
• Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
• Post-translational
  modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
  The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
  Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
• Cellular localizationMyelin membrane. Cytoplasmic side of myelin.

Target information above from: UniProt accession P02686 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4155 Human
  • Entrez Gene: 24547 Rat
  • Omim: 159430 Human
  • SwissProt: P02686 Human
  • SwissProt: P02688 Rat
  • Unigene: 551713 Human
  • Unigene: 63285 Rat

• Alternative names
  GDB antibodyGolli MBP antibodyGolli MBP; myelin basic protein antibody

  Hemopoietic MBP antibodyHMBPR antibodyHUGO antibodyMBP antibodyMBP_HUMAN antibodyMGC99675 antibodyMLD antibodyMyelin A1 Protein antibodyMyelin basic protein antibodyMyelin Deficient antibodyMyelin Membrane Encephalitogenic Protein antibodyOTTHUMP00000163776 antibodyOTTHUMP00000174387 antibodyOTTHUMP00000174388 antibodySHI antibodyShiverer antibodySP antibody

  see all

Anti-Myelin Basic Protein antibody [7D2] images

• 

  Western blot - Myelin Basic Protein antibody [7D2] (ab78157)
  Anti-Myelin Basic Protein antibody [7D2] (ab78157) at 1/10000 dilution + Crude rat spinal cord homogenate
  
  Predicted band size : 21.5 kDa
  Observed band size : 21.5 kDa
  
• 

  Western blot - Myelin Basic Protein antibody [7D2] (ab78157)
  Lane 1 : Anti-Myelin Basic Protein antibody [7G7] (ab78156) at 1/10000 dilution
  Lane 2 : Anti-Myelin Basic Protein antibody [7D2] (ab78157) at 1/10000 dilution
  
  Lane 1 : Crude rat spinal cord homogenate
  Lane 2 : Crude rat spinal cord homogenate
  
  
  Predicted band size : 21.5 kDa
  Observed band size : 18,21.5 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Myelin Basic Protein antibody [7D2] (ab78157)
  ab78157 (red) staining Myelin Basic Protein in rat mixed neuron/glial cultures. Also stained with a chicken antibody to neurofilament NF-L (green). Blue is a DNA stain. Note that ab78157 stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative for Myelin Basic Protein, though the neuronal processes can be seen with the NF-L antibody.
• 

  Flow Cytometry-Anti-Myelin Basic Protein antibody [7D2](ab78157)
  Overlay histogram showing SH-SY5Y cells stained with ab78157 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78157, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.