Anti-Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)

ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30µm thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of 30 images taken with a 20x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.

Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Image courtesy of Dr Pezet, CARD Institute, KCL, London, UK

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)

Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.

Immunohistochemistry (PFA perfusion fixed frozen sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)

ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.

Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).

Image courtesy of Dr Pezet, CARD institute, KCL, London, UK