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Immunohistochemistry (PFA perfusion fixed frozen sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
(enlarge)
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Product Name
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Myelin Basic Protein antibody - Oligodendrocyte Marker
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See all Myelin Basic Protein antibodies (22)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Myelin Basic Protein - Oligodendrocyte Marker
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Immunogen
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Human myelin basic protein isolated from normal human brain.
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Reacts with
(species key)
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Hu, Rat
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Specificity
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This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord.
Grey matter stains weakly. This antibody also stains peripheral nerves.
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Tested applications
(see key)
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IHC-FoFr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/20 - 1/40. (ABC method). We suggest an incubation period of 30 minutes at room temperature. Antigen retrieval is not always necessary but it may increase signal intensity.
IHC-FoFr: 1/1000. See recommended protocol.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Brain and spinal cord.
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Cellular localization
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Cytoplasmic side of myelin.
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Research areas
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Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins
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Relevance
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The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, Whole serum Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Myelin Basic Protein antibody - Oligodendrocyte Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Myelin Basic Protein antibody - Oligodendrocyte Marker images, references, products related to ab2404 and other tools.
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Myelin Basic Protein antibody - Oligodendrocyte Marker images:
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 Immunohistochemistry (PFA perfusion fixed frozen sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30µm thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of 30 images taken with a 20x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates). Image courtesy of Dr Pezet, CARD Institute, KCL, London, UK
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
Human brain (grey matter) formalin fixed paraffin embedded 4 micron tissue sections. No antigen retrieval was performed. ab2404 (1:40) incubated for 30 min and detected using Mouse/Rabbit PolyVue HRP/ DAB Detection System (a 2-step HRP polymer system for enhanced sensitivity). Tissue was treated with peroxidase block and counterstained with Hematoxylin.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
ab2404 at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed description). This image is a composite of several images taken with a 40x objective. No labeling observed following omission of primary antibody. Image recoloured in Photoshop.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates). Image courtesy of Dr Pezet, CARD institute, KCL, London, UK
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References for Myelin Basic Protein antibody - Oligodendrocyte Marker (ab2404)
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This product has been used in:
Clavaguera F et al. Transmission and spreading of tauopathy in transgenic mouse brain. Nat Cell Biol : (2009). IHC-FoFr; Mouse. PubMed: 19503072
If you publish research using ab2404 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Myelin Basic Protein
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