Myelin Basic Protein antibody [SMI-94] (ab24567)

Product Name 

Myelin Basic Protein antibody [SMI-94]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SMI-94] to Myelin Basic Protein

Immunogen 

Recombinant fragment: TADPKNAWQD AHPADPGSRP, corresponding to amino acids 70-89 of Human Myelin Basic Protein
BLAST 'TADPKNAWQD AHPADPGSRP' with or

Reacts with 
(species key)

Hu, Ms, Rat, Dog, Mk, Rb

Specificity 

Ab24567 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections.

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
IHC-P: 1/1000. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr: 1/1000.
WB: 1/1000. Predicted molecular weight: 33 kDa.

Tissues can be fixed in a variety of paraformaldehyde or formaldehyde-containing fixatives. To insure penetration of antibody into myelin it is necessary to pretreat frozen or vibratome sections with cold methanol (30 min) or detergent. Reaction on formalin-fixed, paraffin-embedded sections may be enhanced by autoclaving deparaffinized sections in distilled water for 10 min as described by Shin et al, Meth in Lab Invest,64(#5), p 693-,1991, or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min as described by Evers and Uylings, J Neurosc Meth 72:197, 1997.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic side of myelin.

Research areas 

Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins

Relevance 

The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

The combination of clone SMI-94 with clone SMI-91 and/or 99 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SMI-94

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 0.01M Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for Myelin Basic Protein antibody [SMI-94] images, references, products related to ab24567 and other tools.

Myelin Basic Protein antibody [SMI-94] images:

References for Myelin Basic Protein antibody [SMI-94] (ab24567)

  This product has been used in: (two most recent references)

Sargiannidou I et al. Connexin32 mutations cause loss of function in Schwann cells and oligodendrocytes leading to PNS and CNS myelination defects. J Neurosci 29:4736-49 (2009). IHC-FoFr; Mouse. PubMed: 19369543

Zhang J et al. Bone marrow stromal cells increase oligodendrogenesis after stroke. J Cereb Blood Flow Metab 29:1166-74 (2009). IHC-P; Rat. PubMed: 19384336

See all 3 publication references for this product.


If you publish research using ab24567 please let us know so that we can cite the reference on this datasheet.

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