Anti-Myelin Basic Protein antibody (ab28541)

Overview

• Product nameAnti-Myelin Basic Protein antibodySee all Myelin Basic Protein primary antibodies ...
• Description
  Rabbit polyclonal to Myelin Basic Protein
• SpecificityThis antibody is specific to myelin basic proteins. It may cross react with other isoforms of myelin basic proteins.
• Tested applicationsWB, IP, Sandwich ELISA more details
• Species reactivity
  Reacts with: Cow, Pig
  Predicted to work with: all Mammals
• Immunogen

  Full length myelin basic protein (Cow).

• Positive controlBovine brain extract.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 0.1M Tris acetate. pH 7.0
• Concentration information loading...
• Purification notesThe antibody was affinity purified on agarose with an immuno dominant peptide (PRTPPPSQGKGRGL), which is a universal peptide sequence for all isoforms of mammalian myelin basic proteins.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Adhesion Proteins
  • Membrane Proteins

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Oligodendrocyte marker

Applications

Target

• FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
• Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
• Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
• Sequence similaritiesBelongs to the myelin basic protein family.
• Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
• Post-translational
  modificationsSeveral charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
  The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
  Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
• Cellular localizationMyelin membrane. Cytoplasmic side of myelin.

Target information above from: UniProt accession P02686 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 618684 Cow
  • Entrez Gene: 414286 Pig
  • SwissProt: P02687 Cow
  • SwissProt: P81558 Pig

• Alternative names
  GDB antibodyGolli MBP antibodyGolli MBP; myelin basic protein antibody

  Hemopoietic MBP antibodyHMBPR antibodyHUGO antibodyMBP antibodyMBP_HUMAN antibodyMGC99675 antibodyMLD antibodyMyelin A1 Protein antibodyMyelin basic protein antibodyMyelin Deficient antibodyMyelin Membrane Encephalitogenic Protein antibodyOTTHUMP00000163776 antibodyOTTHUMP00000174387 antibodyOTTHUMP00000174388 antibodySHI antibodyShiverer antibodySP antibody

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Anti-Myelin Basic Protein antibody images

• 

  Sandwich ELISA - Myelin Basic Protein antibody (ab28541)
  Fixed Immobilized Anti-MBP (ab28541): 2 ug/mL, 100 ul/well
  Captured Antigen: Swine MBP , range from 1 to 90 ng/mL
  Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt
• 

  Sandwich ELISA - Myelin Basic Protein antibody (ab28541)
  Immobilized Anti-MBP (ab28541): range from 0.125 to 2 µg/ml, 100 µl/well
  Fixed Captured Antigen: Swine MBP, 500 ng/mL
  Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt