Anti-Myelin Basic Protein antibody (ab28541)

Overview

  • Product nameAnti-Myelin Basic Protein antibodySee all Myelin Basic Protein primary antibodies ...
  • Description
    Rabbit polyclonal to Myelin Basic Protein
  • SpecificityThis antibody is specific to myelin basic proteins. It may cross react with other isoforms of myelin basic proteins.
  • Tested applicationsWB, IP, Sandwich ELISA more details
  • Species reactivity
    Reacts with: Cow, Pig
    Predicted to work with: all Mammals
  • Immunogen

    Full length myelin basic protein (Cow).

  • Positive control
    • Bovine brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab28541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 20 kDa.
IP Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. To be used as capture antibody. Pair with ab77895 - Rabbit polyclonal to Myelin Basic Protein (HRP)

Target

  • FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similaritiesBelongs to the myelin basic protein family.
  • Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications
    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localizationMyelin membrane. Cytoplasmic side of myelin.
  • Target information above from: UniProt accession P02686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • GDB antibody
    • Golli MBP antibody
    • Golli MBP; myelin basic protein antibody
    • Hemopoietic MBP antibody
    • HMBPR antibody
    • HUGO antibody
    • MBP antibody
    • MBP_HUMAN antibody
    • MGC99675 antibody
    • MLD antibody
    • Myelin A1 Protein antibody
    • Myelin basic protein antibody
    • Myelin Deficient antibody
    • Myelin Membrane Encephalitogenic Protein antibody
    • OTTHUMP00000163776 antibody
    • OTTHUMP00000174387 antibody
    • OTTHUMP00000174388 antibody
    • SHI antibody
    • Shiverer antibody
    • SP antibody
    see all

Anti-Myelin Basic Protein antibody images

  • Fixed Immobilized Anti-MBP (ab28541): 2 ug/mL, 100 ul/well
    Captured Antigen: Swine MBP , range from 1 to 90 ng/mL
    Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt
  • Immobilized Anti-MBP (ab28541): range from 0.125 to 2 µg/ml, 100 µl/well
    Fixed Captured Antigen: Swine MBP, 500 ng/mL
    Detection: anti-MBP HRP (ab77895), 0.25 ug/mL in 1% BSA and PBSt

References for Anti-Myelin Basic Protein antibody (ab28541)

ab28541 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab28541.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"