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Myelin Basic Protein (Biotin) (Active) (ab792)

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Overview

  • Product nameMyelin Basic Protein (Biotin) (Active)See all Myelin Basic Protein proteins and peptides ...
  • Expression hostNative
  • ConjugationBiotin

    • Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferConstituents: 0.1M MOP. pH 4.0-4.5
  • Concentration information loading...
  • Conjugation notesBiotinylated. The molecular ratio for biotin:MBP is approximately 3:1.
  • Additional notesMyelin basic protein (MBP) is isolated from bovine brain. It is well documented that MBP is a substrate for many protein kinases including PKC, PKA, and MAPK family (such as ERK1/2). Biotinylated MBP is an excellent substrate for the solid-phase kinase assay system, facilitating the separation of free ATP and the kinases. Biotinylated MBP could enhance the efficiency of immobilization of the substrate on an avidin-coated microplate and the substrate will be readily accessible for the kinase activity through a large molecule avidin as a spacer. We do not sell non-biotinylated MBP.
  • Sequence notesThe protein has a molecular weight of 20 Kd. It is approximately 1 Kd larger than non-biotinylated MBP.
  • Research Areas

    • Applications

  • Application notesAs immobilized substrate for high throughput kinase assay.
    See figure for Western assay.
    Plate assay: Immobilized biotinylated MBP (10 ug/mL, 150uL/well) was phosphorylated with ERK1(0.02 ug/well).
    Kinase ERK1 Biot-MBP P32 transfer activities (Counts/min):
    +ERK1 +MBP =1865 c/min
    -ERK1 +MBP =85 c/min
    +ERK1 -MBP =112 c/min

    • Protein info

    • Alternative names
        GDBGolli MBPGolli MBP; myelin basic protein
        Hemopoietic MBPHMBPRHUGOMBPMBP_HUMANMGC99675MLDMyelin A1 ProteinMyelin basic proteinMyelin DeficientMyelin Membrane Encephalitogenic ProteinOTTHUMP00000163776OTTHUMP00000174387OTTHUMP00000174388SHIShivererSP
      see all
  • FunctionThe classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
  • Tissue specificityMBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
  • Involvement in diseaseNote=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
  • Sequence similaritiesBelongs to the myelin basic protein family.
  • Developmental stageExpression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
  • Post-translational
    modifications    Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
    The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
    Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  • Cellular localizationMyelin membrane. Cytoplasmic side of myelin.

    • Target information above from: UniProt accession P02686 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Myelin Basic Protein (Biotin) (Active) images

    • Western blot - Myelin Basic Protein protein (Biotin) (ab792)
      Western blot - Myelin Basic Protein protein (Biotin) (ab792)

      MBP and Biotinylated MBP was phosphorylated with 0.05ug of active ERK1 with P32 radiolabeled ATP.
      Assay reference: Sanghera JS et al., J Immunol. (1996) 156:4457-4465

      Activity of biotinylated MBP
      Lane a (5 ug of biotinylated MBP): 33682 counts/min
      Lane b (2.5 ug of biotinylated MBP): 18478 counts/min
      Lane c (1.25 ug of biotinylated MBP): 6714 counts/min
      Lane d (0.625 ug of biotinylated MBP): 2889 counts/min
      Lane e (0.36 ug of biotinylated MBP): 1039 counts/min
      Lane f (0.18 ug of biotinylated MBP): 633 counts/min
      Control Lane (0.0 ug of biotinylated MBP): 145 counts/min

      Non-biotinylated MBP Activity
      Lane A (5 ug of biotinylated MBP): 37604 counts/min
      Lane B (2.5 ug of biotinylated MBP): 17824 counts/min
      Lane C (1.25 ug of biotinylated MBP): 8867 counts/min
      Lane D (0.625 ug of biotinylated MBP): 4190 counts/min
      Lane E (0.36 ug of biotinylated MBP): 2248 counts/min
      Lane F (0.18 ug of biotinylated MBP): 1500 counts/min
      Control Lane (0.0 ug of biotinylated MBP): 218 counts/min

    References for Myelin Basic Protein (Biotin) (Active) (ab792)

    ab792 has not yet been referenced specifically in any publications.

    Publishing research using ab792 ? Please let us know so that we can cite the reference in this datasheet

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    Do you have any information on the ratio of biotin molecules for the antibody?

    Thank you for your enquiry. The molecular ratio for biotin:MBP is approximately 3:1. I hope this information helps. Please do not hesitate to contact us if you need anything further.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"