Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)

Overview

• Product nameAnti-Myeloid leukemia factor 1 antibody [3A3]See all Myeloid leukemia factor 1 primary antibodies ...
• Description
  Mouse monoclonal [3A3] to Myeloid leukemia factor 1
• Tested applicationsWB, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length Human Myeloid leukemia factor 1 produced in E.coli (NP_071888).

• Positive controlHEK293T cells transfected with pCMV6-ENTRY Myeloid leukemia factor 1; Human colon adenocarcinoma, ovary adenocarcinoma, pancreas, thyroid carcinoma, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues; COS7 cells transiently transfected by pCMV6-ENTRY Myeloid leukemia factor 1.
• General notesDilute in PBS (pH7.3) before use.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferpH: 7.30
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 1% BSA
• Concentration information loading...
• PurityProtein G purified
• Purification notesab128108 is purified from Mouse ascites fluid by affinity chromatography.
• Clonality Monoclonal
• Clone number3A3
• IsotypeIgG1
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cardiovascular
  • Blood
  • Other

  • Tags & Cell Markers
  • Cell Type Markers
  • Tumor Associated

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionInvolved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest. Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
• Tissue specificityMost abundant in testis, ovary, skeletal muscle, heart, kidney and colon. Low expression in spleen, thymus and peripheral blood leukocytes.
• Involvement in diseaseNote=A chromosomal aberration involving MLF1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with NPM1/NPM.
• Sequence similaritiesBelongs to the MLF family.
• Post-translational
  modificationsPhosphorylation is required for binding to YWHAZ.
• Cellular localizationCytoplasm. Nucleus. In non-hematopoietic cells, resides primarily in the cytoplasm with some punctate nuclear localization. Shuttles between the cytoplasm and nucleus. In hematopoietic cells, located preferentially in the nucleus. Found in the nucleolus when fused to NPM.

Target information above from: UniProt accession P58340 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4291 Human
  • Omim: 601402 Human
  • SwissProt: P58340 Human
  • Unigene: 85195 Human

• Alternative names
  Hls7 antibodyMLF1 antibodyMLF1_HUMAN antibody

  Myelodysplasia myeloid leukemia factor 1 antibodyMyelodysplasia-myeloid leukemia factor 1 antibodyMyeloid leukemia factor 1 antibodymyeloid leukemia factor 1 variant 1 antibodymyeloid leukemia factor 1 variant 2 antibodymyeloid leukemia factor 1 variant 3 antibody

  see all

Anti-Myeloid leukemia factor 1 antibody [3A3] images

• 

  Western blot - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  All lanes : Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108) at 1/2000 dilution
  
  Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
  Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Myeloid leukemia factor 1 cDNA
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 31 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/150 dilution, staining Myeloid leukemia factor 1 in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/150 dilution, staining Myeloid leukemia factor 1 in paraffin-embedded Human ovary adenocarcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/150 dilution, staining Myeloid leukemia factor 1 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/150 dilution, staining Myeloid leukemia factor 1 in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at a dilution of 1/150, staining Myeloid leukemia factor 1 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/150 dilution, staining Myeloid leukemia factor 1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/150 dilution, staining Myeloid leukemia factor 1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at 1/100 dilution, staining Myeloid leukemia factor 1 COS7 cells transiently transfected with pCMV6-ENTRY Myeloid leukemia factor 1 by Immunofluorescence.
• 

  Flow Cytometry - Anti-Myeloid leukemia factor 1 antibody [3A3] (ab128108)
  ab128108, at a dilution of 1/100, staining Myeloid leukemia factor 1 in HEK293T cells transfected with either pCMV6-ENTRY Myeloid leukemia factor 1 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry.