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Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)

Q&A (12)

Overview

  • Product nameMyeloperoxidase (MPO) Activity Assay Kit (Fluorometric)See all Myeloperoxidase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Sensitivity
    > 0.001 mU/well
  • Species reactivity
    Reacts with: all Mammals
  • Product overview

    In Abcam's Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric), MPO catalyzes the production of NaClO from H2O2 and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 µU per well
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.

  • Tested applicationsFunctional Studies more details

Properties

  • FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
  • Cellular localizationLysosome.

    • Target information above from: UniProt accession P05164 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        MPOMyeloperoxidase heavy chainPERM_HUMAN

    Applications

    Our Abpromise guarantee covers the use of ab111749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) images

    • Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
      Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
      Standard Curve using this kit protocol (Ex/Em = 485/525 nm)
    • Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
      Functional Studies - Myeloperoxidase (MPO) Fluorometric Activity Assay Kit (ab111749)
      Positive Control using this kit protocol (Ex/Em = 485/525 nm)

    Protocols

    References for Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)

    ab111749 has not yet been referenced specifically in any publications.

    Publishing research using ab111749 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab111749 Submit a Question for ab111749

    Displaying 1 - 10 of 12 results for Abreviews and Q&A

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    Hi,
    I will be using your product ab111749 MPO activity assay kit for mouse organ samples.
    Since we have many animals to sacrifice, we wonder if we can keep organ samples in freezer for using this MPO activity kit.
    I am considering to necros...

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    Thank you for your enquiry regarding ab111749.

    We have always used with fresh samples only with this assay. We know of scientists who did store the homogenates in aliquots at -80°C for a short while before use but since we don’t oursel...

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    Customer kindly contacted us regarding MPO activity assay kit (Fluorometric) with a couple questions.
    1. Can tissue samples homogenized in assay buffer be frozen at -20 or -80? If so, what buffer conditions are appropriate (protease inhibitors, etc.)<...

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    The homogenized samples can be stored in aliquots at -80°C, w/o addition of any other components to the assay buffer. It would be preferable though to use fresh samples.
    “V” is the volume of the sample you add to the wells in step 4.1...

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    ‘Can this MPO assay kit (http://www.abcam.com/Myeloperoxidase-MPO-Activity-Assay-Kit-Fluorometric-ab111749.html) be used for rat tissue homogenates, or is it only for human samples?

    This kit can be used with all mammalian samples including rats.

    I have updated our datasheet accordingly.

    How will EDTA inhibit this assay?

    This is an enzymatic activity assay. Enzymes need some ions/cofactors to function and EDTA being a chelator will chelate these co-factors (specifically Mg++ and Ca++), leading to the enzyme becoming non-functional.

    What is the concentration of the MPO positive control for my lot?

    Thank you for your inquiry and your patience with this reply.

    I heard back from the supplying laboratory that the concentration of the positive control MPO does not vary from lot to lot. But unfortunately, they could not disclose the concentrat...

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    Should a black plate with clear bottom wells or opaque bottom wells be used with this kit?


    For this assay kit, the client can either use a black opaque bottom plate or, for even more efficiency, use a white opaque bottom plate. One would not use a clear transparent plate.

    Should a black plate with clear bottom wells or opaque bottom wells be used with this kit?



    I am awaiting a reply from the lab which supplies this kit. However I believe you can use a plate with either clear well bottoms or opaque well bottoms to detect fluorometric signal. Although you would use a clear bottom well if you need to visu...

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    Inquiry: Dear Technical Support team: We are interested in measuring MPO activity in mouse liver tissue. I read that Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) is compatible with tissue extract. Is this kit compatible wit...

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    Thank you for contacting us.

    I would recommend using fresh tissue samples.

    Other peroxidases will not be able to produce the same intermediates required for this assay. Therefore it is specific for MPO.


    I hope this informat...

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    How much tissue should I use? Do I need to do anything in particular to prepare muscle tissue?

    Thank you for contacting Abcam regarding ab111749.


    For tissues, start with about 10 mg tissue with 400 ul of assay buffer for homogenization. There are no specific guidelines recommended for muscle tissue in particular.


    I hope...

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    protocol booklet missing for ab111749

    Merci de nous avoir contactés.
    Veuillez trouver ci-joint le protocole pour le kit ab111749.
    Le lien vers ce livret était manquant sur la fiche technique, ceci sera corrigé dans les prochaines heures.
    Nous sommes désolés pour cette erreur. <...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"