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In Abcam's Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric), MPO catalyzes the production of NaClO from H2O2 and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 µU per well
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Myeloperoxidase (MPO) is a peroxidase enzyme (EC 220.127.116.11) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent.
|Assay Buffer||WM||1 x 25ml|
|Fluorescein Standard (1 mM)||Yellow cap||1 x 50µl|
|MPO Positive Control||Purple cap||1 vial|
|MPO Probe||Red cap||1 x 200µl|
|MPO Substrate Stock||Blue cap||1 x 50µl|
Our Abpromise guarantee covers the use of ab111749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.
ab111749 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"