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Myeloperoxidase (MPO) Assay Kit (Colorimetric) (ab105136)

Q&A (1)

Overview

  • Product nameMyeloperoxidase (MPO) Assay Kit (Colorimetric)See all Myeloperoxidase kits ...
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Product overview

    Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly expressed in neutrophil granulocytes. It is a lysosomal protein stored in the azurophilic granules of the neutrophil. Myeloperoxidase contains a heme pigment which causes its green color in secretions rich in neutrophils, such as pus and some forms of mucus. Myeloperoxidase catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). Myeloperoxidase also oxidizes tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In ab105136, the HClO produced from H2O2 and Cl- is reacted with taurine to generate the taurine chloramine, which subsequently reacts with the TNB2- probe to eliminate color (lambda = 412 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of Myeloperoxidase. This kit can be used to detect Myeloperoxidase as low as 0.05 mU per well.

  • Notes

    Store the kit at -20 °C protected from light. Allow the Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol prior to performing the assay.

  • Tested applicationsFunctional Studies more details

Properties

  • FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
  • Cellular localizationLysosome.

    • Target information above from: UniProt accession P05164 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        MPOMyeloperoxidase heavy chainPERM_HUMAN

    Applications

    Our Abpromise guarantee covers the use of ab105136 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Myeloperoxidase (MPO) Assay Kit (Colorimetric) images

    • Functional Studies - (ab105136)
      Functional Studies - (ab105136)

    Protocols

    References for Myeloperoxidase (MPO) Assay Kit (Colorimetric) (ab105136)

    ab105136 has not yet been referenced specifically in any publications.

    Publishing research using ab105136 ? Please let us know so that we can cite the reference in this datasheet

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    Probe tube was spun in a balanced microcentrifuge and the bottom broke off. Would like to receive another probe.

    Thank you very much for you call today and for letting us know about the problem with this tube of probe.

    As we discussed, please send me the original order or PO number, and I'll see if we can just send you a replacement vial of the probe.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"