Anti-Myeloperoxidase antibody (ab139748)
Key features and details
- Rabbit polyclonal to Myeloperoxidase
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
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Overview
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Product name
Anti-Myeloperoxidase antibody
See all Myeloperoxidase primary antibodies -
Description
Rabbit polyclonal to Myeloperoxidase -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab208670.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse Myeloperoxidase aa 650 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: P11247 -
Positive control
- This antibody gave a positive signal in Mouse Bone Marrow and Mouse Spleen tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal spleen.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 81 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 81 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 17523 Mouse
- Entrez Gene: 303413 Rat
- SwissProt: P11247 Mouse
- Unigene: 4668 Mouse
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Alternative names
- 84 kDa myeloperoxidase antibody
- 89 kDa myeloperoxidase antibody
- EC 1.11.1.7 antibody
see all
Images
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IHC image of Myeloperoxidase staining in Mouse normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139748, 1µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-Myeloperoxidase antibody (ab139748) at 1 µg/ml
Lane 1 : Mouse Bone Marrow Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa (possible cleavage fragment)
Exposure time: 30 secondsThe band observed at 64 kDa could potentially be a cleaved form of Myeloperoxidase due to the presence of both a 15 amino acid signal peptide and a 123 amino acid propeptide.
The band observed at 48 kDa could also represent the Myeloperoxidase heavy chain.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab139748 has been referenced in 11 publications.
- Kreiss L et al. Label-free analysis of inflammatory tissue remodeling in murine lung tissue based on multiphoton microscopy, Raman spectroscopy and machine learning. J Biophotonics 15:e202200073 (2022). PubMed: 35611635
- Zemskov EA et al. NF-κB-dependent repression of Sox18 transcription factor requires the epigenetic regulators histone deacetylases 1 and 2 in acute lung injury. Front Physiol 13:947537 (2022). PubMed: 35991176
- Lin ZC et al. Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation. Theranostics 11:4567-4584 (2021). PubMed: 33754014
- Reis-Mendes A et al. Inflammation as a Possible Trigger for Mitoxantrone-Induced Cardiotoxicity: An In Vivo Study in Adult and Infant Mice. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34073506
- Lv C et al. CTRP3 ameliorates cerulein-induced severe acute pancreatitis in mice via SIRT1/NF-?B/p53 axis. Biosci Rep 40:N/A (2020). PubMed: 32219332