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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148)](/ps/datasheet/images/ab16148.jpg)
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Product Name
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MyoD1 antibody [5.2F]
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See all MyoD1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [5.2F] to MyoD1
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Immunogen
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Synthetic peptide: LLSPPLRDID LTGPDGSLCS FETADDFYDD PCFDSPDLRF FEDLDPRLVH corresponding to N terminal amino acids 3-56 of Mouse Myogenin D1.
BLAST 'LLSPPLRDID LTGPDGSLCS FETADDFYDD PCFDSPDLRF FEDLDPRLVH ' with  or 
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Reacts with
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Hu, Ms, Rat, Chk
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Predicted to react
(species key)
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Cow, Pig, Shp
(Due to sequence homology)
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Specificity
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This antibody does not cross react with Myogenin, Myf5, or Myf6.
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Tested applications
(see key)
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ELISA, EM, ICC/IF, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Electron Microscopy: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml.
IHC-Fr: Use at a concentration of 2 - 4 µg/ml.
IP: Use 2µg/mg of protein lysate.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rhabdomyosarcoma.
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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5.2F
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG2a
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.08% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.37 - 0.37 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MyoD1 antibody [5.2F] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MyoD1 antibody [5.2F] images, references, products related to ab16148 and other tools.
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MyoD1 antibody [5.2F] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148)
Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.
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Search PubMed (MEDLINE) for references to MyoD1
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MyoD1 antibody [5.2F] - more information
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Customer reviews (feedback) regarding MyoD1 antibody [5.2F] |
Customer FAQs regarding MyoD1 antibody [5.2F] |
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MyoD1 antibody [5.2F] - related products:
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MyoD1 antibody [5.2F] - other tools:
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Contact Abcam with a Technical Enquiry about MyoD1 antibody [5.2F] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148)](/index.html?datasheet=16148#ab16148.jpg)
