![Sandwich ELISA - MyoD1 antibody [5.2F] (ab16148) image](#MyoD1-Primary-antibodies-ab16148-5.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148) image](#ab16148.jpg)
![Immunocytochemistry/ Immunofluorescence - MyoD1 antibody [5.2F] (ab16148) image](#MyoD1-Primary-antibodies-ab16148-4.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Overview
Product name
Anti-MyoD1 antibody [5.2F]
See all MyoD1 products (10) ...
Description
Mouse monoclonal [5.2F] to MyoD1
Specificity
This antibody does not cross react with Myogenin, Myf5, or Myf6.
Tested applications
ICC/IF, Sandwich ELISA, ELISA, IF, IP, IHC-P, IHC-Fr, Electron Microscopy, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Predicted to work with
Sheep, Cow, Pig
Immunogen
Synthetic peptide: LLSPPLRDIDLTGPDGSLCSFETADDFYDDPCFDSPDLRF FEDLDPRLVHVGAL, corresponding to N terminal amino acids 3-56 of Mouse Myogenin D1.
LLSPPLRDID LTGPDGSLCS FETADDFYDD PCFDSPDLRF FEDLDPRLVH VGAL
Positive control
Rhabdomyosarcoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.08% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
5.2F
Myeloma
Sp2/0-Ag14
Isotype
IgG2a
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
sELISA: Use a concentration of 5 µg/ml Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). (For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection.)
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: Use at 2 µg/mg of lysate.
IHC-P: Use a concentration of 2 - 4 µg/ml.
IHC-Fr: Use a concentration of 2 - 4 µg/ml.
EM: Use at an assay dependent dilution.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 35 kDa.
Target
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15172
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Database links
- Entrez Gene: 374048 Chicken
- Entrez Gene: 281938 Cow
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Entrez Gene: 407604 Pig
- Entrez Gene: 337868 Rat
- Omim: 159970 Human
- SwissProt: P16075 Chicken
see all
Anti-MyoD1 antibody [5.2F] images:
Sandwich ELISA - MyoD1 antibody [5.2F] (ab16148)
![Sandwich ELISA - MyoD1 antibody [5.2F] (ab16148)](/ps/datasheet/images/16/ab16148/MyoD1-Primary-antibodies-ab16148-5.jpg)
Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab16148)](/ps/datasheet/Images/16/ab16148/ab16148.jpg)
Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.
Immunocytochemistry/ Immunofluorescence - MyoD1 antibody [5.2F] (ab16148)
![Immunocytochemistry/ Immunofluorescence - MyoD1 antibody [5.2F] (ab16148)](/ps/datasheet/images/16/ab16148/MyoD1-Primary-antibodies-ab16148-4.jpg)
ab16148 staining MyoD1 in rat differentiated skeletal muscle cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde and then blocked using 2% serum for 1 hour. Samples were then incubated with primary antibody at 1/200 for 8 hours. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. DAPI was used to stain the cell nuclei (blue).
Image courtesy of an Abreview submitted by Mal Niladri.
Protocols
References for Anti-MyoD1 antibody [5.2F] (ab16148)
This product has been referenced in:
- Sarraf CEet al. In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif 44:99-108 (2011).Read more (PubMed: 21199014) »
- Horton JAet al. Physeal bystander effects in rhabdomyosarcoma radiotherapy: experiments in a new xenograft model. Sarcoma 2011:815190 (2011). IHC-P; Mouse.Read more (PubMed: 21559211) »
See all 6 publications for this product
Publishing research using ab16148? Please let us know so that we can cite the reference in this datasheet
![MyoD1 antibody [5.2F] for WB in Mouse (16148)](/ps/Reviews/Images/ab9466_47851.jpg)
![Myogenin D1 antibody [5.2F] for ICC/IF in Rat (16148)](/ps/Reviews/Images/ab6194_22500.jpg)
![MyoD1 antibody [5.2F] for WB in Mouse (16148)](/ps/datasheet/images/16/ab16148/MyoD1-Primary-antibodies-ab16148-3.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"