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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-MyoD1 antibody [5.2F], prediluted
See all MyoD1 products (10) ...
Description
Mouse monoclonal [5.2F] to MyoD1, prediluted
Specificity
This antibody is specific to a 45 kD protein. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.
Tested applications
IHC-Frmore details
Cross reactivity
Reacts with
Mouse
Immunogen
BALB/C mice were injected with a recombinant mouse MyoD1 protein.
Epitope
This antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1.
Positive control
Tonsil
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: Inert stabilizer
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
5.2F
Isotype
IgG2a
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
Target
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15172
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Database links
- Entrez Gene: 17927 Mouse
- SwissProt: P10085 Mouse
- Unigene: 1526 Mouse
References for Anti-MyoD1 antibody [5.2F], prediluted (ab7272)
ab7272 has not yet been referenced specifically in any publications.
Publishing research using ab7272? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"