![Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.8A] (ab49476) image](#ab49476_1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-MyoD1 antibody [5.8A]
See all MyoD1 products (10) ...
Description
Mouse monoclonal [5.8A] to MyoD1
Tested applications
IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant wild type murine MyoD1 protein.
Positive control
Human Rhabdomyosarcoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Tissue culture supernatant
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
5.8A
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.8A] (ab49476)
![Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.8A] (ab49476) image](/ps/datasheet/Images/49/ab49476/ab49476_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab49476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/20 - 1/50.(Optimum fixative; Zamboni's for 10 minutes at 25°C.)
IHC-P: 1/20 - 1/50.(Heat mediated antigen retrieval using 1mM EDTA (pH8.0) is recommended before commencing with IHC staining protocol.I)
Application notes
Is unsuitable for or WB.
Target
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15172
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Database links
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Omim: 159970 Human
- SwissProt: P15172 Human
- SwissProt: P10085 Mouse
- Unigene: 181768 Human
- Unigene: 1526 Mouse
Anti-MyoD1 antibody [5.8A] images:
Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.8A] (ab49476)
Ab49476, at a dilution of 1/20, staining MyoD1 in paraffin sections of human rhabdomyosarcoma by Immunohistochemistry.
Note staining of a proportion of tumor cell nuclei.
References for Anti-MyoD1 antibody [5.8A] (ab49476)
ab49476 has not yet been referenced specifically in any publications.
Publishing research using ab49476? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"