|
|
|
|
Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab788)
![Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab788)](/ps/datasheet/images/ab788_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
MyoD1 antibody [5.8A]
|
| |
See all MyoD1 antibodies (9)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [5.8A] to MyoD1
|
|
Immunogen
|
BALB/C mice were injected with a recombinant mouse MyoD1 protein.
|
|
Reacts with
(species key)
|
Ms
|
|
Specificity
|
This antibody is specific to an epitope of amino acids 3-56 in the N-terminus of the 45 kDa mouse MyoD1 protein.
This antibody does not react with myogenin, Myf5 or Myf6.
MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.
|
|
Tested applications
(see key)
|
IHC-Fr, IHC-P
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC: This antibody may be diluted to a titer of 1:20-1:50 in an ABC method.
Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.
Formalin fixed paraffin embedded tissue sections require heat mediated antigen retrieval with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
|
|
Relevance
|
MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 17927 (Mouse)
|
| |
|
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
5.8A
|
|
Isotype
|
IgG2a
|
|
Purity
|
Tissue culture supernatant
|
|
Storage buffer
|
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MyoD1 antibody [5.8A] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for MyoD1 antibody [5.8A] images, references, products related to ab788 and other tools.
|
| |
MyoD1 antibody [5.8A] images:
|
 Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab788)
Ab788 at a dilution of 1/200 staining mouse embryonic extraocular muscles by Immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 8 hours and then detected using a Alexa-Fluor ® 568 labeled goat anti-mouse antibody. This image is courtesy of an Abreview by Amanda Evans submitted on 3 February 2006.
See Abreview
|
| |
| |
|
Search PubMed (MEDLINE) for references to MyoD1
|
| |
MyoD1 antibody [5.8A] - more information
|
Customer reviews (feedback) regarding MyoD1 antibody [5.8A] |
Customer FAQs regarding MyoD1 antibody [5.8A] |
Protocols for MyoD1 antibody [5.8A] |
Price and availability of products related to MyoD1 antibody [5.8A] |
| |
MyoD1 antibody [5.8A] - related products:
|
|
|
| |
MyoD1 antibody [5.8A] - other tools:
|
Contact Abcam with a Technical Enquiry about MyoD1 antibody [5.8A] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Paraffin-embedded sections) - MyoD1 antibody [5.2F] (ab788)](/index.html?datasheet=788#ab788_1.jpg)
