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Western blot - MyoD1 antibody (ab64159)
(enlarge)
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Product Name
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MyoD1 antibody
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See all MyoD1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MyoD1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MyoD1. (Peptide available as ab66473.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Pig, RMk
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following Tissue Lysates:
Human Heart, Mouse Skeletal Muscle, Mouse Heart
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.40 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MyoD1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MyoD1 antibody images, references, products related to ab64159 and other tools.
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MyoD1 antibody images:
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 Western blot - MyoD1 antibody (ab64159)
All lanes : MyoD1 antibody (ab64159) at 1 µg/ml
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 34 kDa
Observed band size : 34 kDa
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Search PubMed (MEDLINE) for references to MyoD1
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MyoD1 antibody - more information
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Customer reviews (feedback) regarding MyoD1 antibody |
Customer FAQs regarding MyoD1 antibody |
Protocols for MyoD1 antibody |
Price and availability of products related to MyoD1 antibody |
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MyoD1 antibody - related products:
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MyoD1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MyoD1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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