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Western blot - MyoD1 (phospho S200) antibody (ab75909)
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Product Name
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MyoD1 (phospho S200) antibody
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See all MyoD1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MyoD1 (phospho S200)
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Immunogen
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Synthetic phosphopeptide derived from human MyoD1 around the phosphorylation site of serine 200 (A-S-SP-P-R).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab75909 detects endogenous levels of MyoD1 only when phosphorylated at serine 200.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Extracts from HeLa cells treated with vanadate (1mg/ml, 30min).
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Cellular localization
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Nuclear
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Research areas
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Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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MyoD1 belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. Myod1 is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
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MyoD1 (phospho S200) antibody (ab75909) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab75909 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MyoD1 (phospho S200) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MyoD1 (phospho S200) antibody images, references, products related to ab75909 and other tools.
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MyoD1 (phospho S200) antibody images:
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 Western blot - MyoD1 (phospho S200) antibody (ab75909)
All lanes : MyoD1 (phospho S200) antibody (ab75909) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, un-treated
Lane 2 : Extracts from HeLa cells treated with vanadate (1mg/ml, 30min)
Predicted band size : 35 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to MyoD1
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MyoD1 (phospho S200) antibody - more information
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Customer reviews (feedback) regarding MyoD1 (phospho S200) antibody |
Customer FAQs regarding MyoD1 (phospho S200) antibody |
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MyoD1 (phospho S200) antibody - related products:
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MyoD1 (phospho S200) antibody - other tools:
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Contact Abcam with a Technical Enquiry about MyoD1 (phospho S200) antibody |
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