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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-MyoD1 (phospho S200) antibody
See all MyoD1 products (10) ...
Description
Rabbit polyclonal to MyoD1 (phospho S200)
Specificity
ab75909 detects endogenous levels of MyoD1 only when phosphorylated at serine 200.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human MyoD1 around the phosphorylation site of serine 200 (A-S-SP-P-R).
Positive control
Extracts from HeLa cells treated with vanadate (1mg/ml, 30min).
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab75909 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Western blot - MyoD1 (phospho S200) antibody (ab75909)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab75909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 35 kDa.
Target
Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP15172
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
Database links
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Entrez Gene: 337868 Rat
- Omim: 159970 Human
- SwissProt: P15172 Human
- SwissProt: P10085 Mouse
- SwissProt: A0JPK9 Rat
- SwissProt: Q02346 Rat
see all
Anti-MyoD1 (phospho S200) antibody images:
Western blot - MyoD1 (phospho S200) antibody (ab75909)
All lanes : Anti-MyoD1 (phospho S200) antibody (ab75909) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, un-treated
Lane 2 : Extracts from HeLa cells treated with vanadate (1mg/ml, 30min)
Predicted band size : 35 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
References for Anti-MyoD1 (phospho S200) antibody (ab75909)
ab75909 has not yet been referenced specifically in any publications.
Publishing research using ab75909? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"