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Anti-Myofibroblasts antibody [PR 2D3] (ab20255)

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Overview

Product name

Anti-Myofibroblasts antibody [PR 2D3]

Description

Mouse monoclonal [PR 2D3] to Myofibroblasts

Tested applications

IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Tissue / cell preparation of normal colorectal mucosa (Human).

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

PR 2D3

Myeloma

NS0

Isotype

IgG1

Light chain type

unknown

Applications

Show applications key

Our Abpromise guarantee covers the use of ab20255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • IHC-Fr

     

Application notes

IHC-Fr, Acetone Fixed: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

The antibody reacts with a cell membrane component of cells in the pericrypt sheath, with smooth muscle and with myofibroblasts. The gold standard for identification of myofibroblasts.

References for Anti-Myofibroblasts antibody [PR 2D3] (ab20255)

This product has been referenced in:

  • Kirkland SC & Henderson K Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells. J Cell Sci 114:2055-64 (2001).Read more (PubMed: 11493641) »
  • Richman PI  et al. Colonic pericrypt sheath cells: characterisation of cell type with new monoclonal antibody. J Clin Pathol 40:593-600 (1987).Read more (PubMed: 3301906) »

See all 2 publications for this product

Publishing research using ab20255? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"