Overview
- Product nameAnti-Myogenin antibody [EPR4789]See all Myogenin primary antibodies ...
- DescriptionRabbit monoclonal [EPR4789] to Myogenin
- Tested applicationsWB, IHC-P, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to residues in Human Myogenin.
- Positive controlC2C12 and Human fetal muscle lysates; Human rhabdomyosarcoma tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR4789
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab124800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa). |
| IHC-P | IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
| Flow Cyt | Flow Cyt: 1/10 - 1/100. |
Target
- FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
- Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Cellular localizationNucleus.
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Database links
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 29148 Rat
- Omim: 159980 Human
- SwissProt: P15173 Human
- SwissProt: P12979 Mouse
- SwissProt: P20428 Rat
- Unigene: 2830 Human
- Unigene: 16528 Mouse
- Unigene: 9465 Rat
see all
Target information above from: UniProt accession
P15173
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- bHLHc3 antibodycb553 antibodyClass C basic helix-loop-helix protein 3 antibody
- MYF 4 antibodyMyf-4 antibodyMYF4 antibodyMYOG antibodyMYOG_HUMAN antibodyMyogenic factor 4 antibodyMyogenic factor 4 myogenin antibodyMyogenin antibodyMyogenin myogenin factor 4 antibodyOTTHUMP00000039094 antibody
see all
Anti-Myogenin antibody [EPR4789] images
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![Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)](http://a.abcam.com/ps/datasheet/images/124/ab124800/Myogenin-Primary-antibodies-ab124800-1.jpg)
Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 1/1000 dilution
Lane 1 : C2C12 lysates
Lane 2 : Human fetal muscle lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size : 25 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)](http://a.abcam.com/ps/datasheet/images/124/ab124800/Myogenin-Primary-antibodies-ab124800-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.
References for Anti-Myogenin antibody [EPR4789] (ab124800)
ab124800 has not yet been referenced specifically in any publications.
