Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Myosin light chain (phospho S20) antibody
See all Myosin light chain products (4) ...
Description
Rabbit polyclonal to Myosin light chain (phospho S20)
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS20 of human myosin light chain. Reactivity with non-phosphorylated human myosin light chain is less than 1% by ELISA. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences. Cross reactivity is expected.
Tested applications
ICC/IF, WB, IHC-FoFr, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis, Caenorhabditis elegans
Predicted to work with
Bird, all Mammals
Immunogen
Human Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27).
KKRPQRATSpNVFAMFD
Positive control
Cardiac myocytes.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Contractility >> Contractile Proteins >> Myosins
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
-
Western blot - Myosin light chain (phospho S20) antibody (ab2480)
(enlarge)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin light chain (phospho S20) antibody (ab2480)
(enlarge)
-
Immunohistochemistry (PFA perfusion fixed frozen sections) - Myosin light chain (phospho S20) antibody (ab2480)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: 1/1000 - 1/5000. Predicted molecular weight: 20 kDa.We recommend using BSA for blocking when using phospho-specific antibodies.
IHC-FoFr: Use a concentration of 3 mg/ml. PubMed: 19279134Trichloroacetic acid was used for tissue fixation.
IP
IP: 1/100.IP: 1/100.
IHC-P: Use at an assay dependent concentration.
Target
Relevance
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
Cellular localization
Cytoplasmic
Alternative names
- A1 catalytic antibody
- A2 catalytic antibody
- Alkali myosin light chain 1 antibody
see all
Database links
- Entrez Gene: 10398 Human
- Entrez Gene: 85366 Human
- Entrez Gene: 98932 Mouse
- Entrez Gene: 228785 Mouse
- Entrez Gene: 296313 Rat
- Entrez Gene: 117558 Rat
- Omim: 606566 Human
- Omim: 609905 Human
see all
Anti-Myosin light chain (phospho S20) antibody images:
Western blot - Myosin light chain (phospho S20) antibody (ab2480)
Predicted band size : 20 kDa
Rabbit polyclonal to phospho Myosin Light Chain phospho Myosin Light Chain (Ser 20) (ab2480) used at a 1/5000 dilution to detect myosin light chain by Western blot. Either 13 ug (lanes 1-3) or 20 ug (lanes 4-7) of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA treated:
Lane 1 : untreated 45 min
Lane 2 : CLA 50 nM 45 min
Lane 3 : CLA 100 nM 45 min
Lane 4 : A21 untreated 45 min
Lane 5 : A22 A23187 5 min
Lane 6 : A23 A23187 15 min
Lane 7 : A24 A23187 60 min
After washing, a 1/5,000 dilution of HRP conjugated Goat-anti-Rabbit IgG (ab7090) was used as secondary.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin light chain (phospho S20) antibody (ab2480)
IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Myosin light chain (phospho S20) antibody (ab2480)
ab2480 staining Myosin light chain (phospho S20) in Xenopus laevis embryos by immunohistochemistry (PFA-perfusion fixed frozen tissue sections). The tissue sections were fixed in 2% TCA for 30 minutes and washed with PBST (PBS+0.3% TritonX 100) for 30 minutes. Samples were then blocked in 10% normal goat serum (NGS) for 1 hour at room temperature and incubated with the primary antibody. A Cy5 labelled Goat polyclonal to rabbit IgG was used as secondary.
The arrows shows that depleting E-cadherin does not have any effect on the expression of apical pMLC.
Image from Tao Shijie et. al., Development 136, 1327-1338 (2009), Fig 7G.
References for Anti-Myosin light chain (phospho S20) antibody (ab2480)
This product has been referenced in:
- Harrell JR & Goldstein B Internalization of multiple cells during C. elegans gastrulation depends on common cytoskeletal mechanisms but different cell polarity and cell fate regulators. Dev Biol 350:1-12 (2011). ICC/IF; Caenorhabditis elegans.Read more (PubMed: 20875815) »
- Barnhart EL et al. An Adhesion-Dependent Switch between Mechanisms That Determine Motile Cell Shape. PLoS Biol 9:e1001059 (2011). ICC/IF; Fish.Read more (PubMed: 21559321) »
See all 11 publications for this product
Publishing research using ab2480? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"