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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)
Product partner
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab82255? Please let us know so that we can cite the reference in this datasheet
ab82255 has been referenced in 1 publications.
- Sharifpoor S et al. Functional characterization of human coronary artery smooth muscle cells under cyclic mechanical strain in a degradable polyurethane scaffold. Biomaterials 32:4816-29 (2011). WB; Human. PubMed: 21463894
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)](/ps/datasheet/images/82/ab82255/Myosin-smooth-muscle-heavy-chain-1-and-2-Primary-antibodies-ab82255-3.jpg)
Western blot - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255) at 1/250 dilution + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 227 kDa
Observed band size : 235 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)](/ps/datasheet/images/82/ab82255/Myosin-smooth-muscle-heavy-chain-1-and-2-Primary-antibodies-ab82255-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)
IHC image of ab82255 staining in human artery formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82255, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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