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Product Name 

Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13]

 

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Product type 

Primary antibodies

Description 

Mouse monoclonal [MS-13] to Myosin, smooth muscle heavy chain 1 and 2

Immunogen 

Rabbit muscle myosin.

Reacts with 
(species key)

Hu, Rat, Rb

Specificity 

No cross reactivity with other proteins.

Tested applications 
(see key)

ICC, IHC-Fr, IHC-P, WB


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Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 0.5 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.5 - 1 µg/ml. Use formalin or acetone fixed tissues.
WB: Use at a concentration of 0.25 - 0.5 µg/ml. Predicted molecular weight: 227 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin

Relevance 

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

 

Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4629    (Human)
24582    (Rat)

Omim

    

160745    (Human)

SwissProt

    

P35749    (Human)
Q63862    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

MS-13

Isotype 

IgG1

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1.2% Sodium acetate, PBS, 2mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified by goat anti mouse IgG affinity chromatography.

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

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