![Western blot - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255) image](#Myosin-smooth-muscle-heavy-chain-1-and-2-Primary-antibodies-ab82255-3.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255) image](#Myosin-smooth-muscle-heavy-chain-1-and-2-Primary-antibodies-ab82255-4.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
Overview
Product name
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13]
See all Myosin, smooth muscle heavy chain 1 and 2 products (7) ...
Description
Mouse monoclonal [MS-13] to Myosin, smooth muscle heavy chain 1 and 2
Specificity
No cross reactivity with other proteins.
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Rabbit, Chicken, Human
Immunogen
Human uterus smooth muscle extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1.2% Sodium acetate, 2mg/ml BSA
Concentration
Concentration information loading...
Purification notes
Purified by goat anti mouse IgG affinity chromatography.
Clonality
Monoclonal
Clone number
MS-13
Isotype
IgG1
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab82255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 - 0.5 µg/ml.Predicted molecular weight: 227 kDa.
IHC-P: Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr: Use a concentration of 0.5 - 1 µg/ml.(Formalin or acetone fixed tissues.)
Target
Function
Muscle contraction.
Tissue specificity
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
Involvement in disease
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
Sequence similarities
Contains 1 IQ domain.
Contains 1 myosin head-like domain.
Domain
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Cellular localization
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
Target information above from: UniProt accessionP35749
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FAA4 antibody
- SM1 antibody
- SM2 antibody
see all
Database links
- Entrez Gene: 4629 Human
- Omim: 160745 Human
- SwissProt: P35749 Human
- Unigene: 460109 Human
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] images:
Western blot - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)
![Western blot - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)](/ps/datasheet/images/82/ab82255/Myosin-smooth-muscle-heavy-chain-1-and-2-Primary-antibodies-ab82255-3.jpg)
Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255) at 1/250 dilution + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 227 kDa
Observed band size : 235 kDa (why is the actual band size different from the predicted?)
Exposure time : 90 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)](/ps/datasheet/images/82/ab82255/Myosin-smooth-muscle-heavy-chain-1-and-2-Primary-antibodies-ab82255-4.jpg)
IHC image of ab82255 staining in human artery formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82255, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [MS-13] (ab82255)
This product has been referenced in:
- Sharifpoor Set al. Functional characterization of human coronary artery smooth muscle cells under cyclic mechanical strain in a degradable polyurethane scaffold. Biomaterials 32:4816-29 (2011). WB; Human.Read more (PubMed: 21463894) »
See 1 publication for this product
Publishing research using ab82255? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"