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Product Name
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Myosin, smooth muscle heavy chain 1 and 2 antibody [SM-M10] - BSA and Azide free
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See all Myosin, smooth muscle heavy chain 1 and 2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [SM-M10] to Myosin, smooth muscle heavy chain 1 and 2 - BSA and Azide free
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Immunogen
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Crude Human uterus extract.
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Reacts with
(species key)
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Hu, Cow
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Specificity
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This antibody reacts with Myosin, smooth muscle heavy chain 1 (205kDa) and 2 (200kDa) isoform.
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Tested applications
(see key)
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ICC/IF, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2 - 4 µg/ml. Staining of formalin-fixed tissues requires digesting sections with trypsin at 1mg/ml PBS, 10 min at 37°C, followed by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Uterus.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin
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Relevance
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SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.
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Alternative names Myosin, smooth muscle heavy chain 1 and 2 antibody [SM-M10] - BSA and Azide free (ab81031) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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SM-M10
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 10mM PBS, pH 7.4
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Purification notes
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Purified from the ascites fluid by Protein G chromatography.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Search PubMed (MEDLINE) for references to Myosin, smooth muscle heavy chain 1 and 2
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