Myosin, smooth muscle heavy chain 1 and 2 antibody [SPM201], prediluted (ab17811)

Product Name 

Myosin, smooth muscle heavy chain 1 and 2 antibody [SPM201], prediluted

Product type 

Primary antibodies

Description 

Mouse monoclonal [SPM201] to Myosin, smooth muscle heavy chain 1 and 2, prediluted

Immunogen 

Crude human uterus extract

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions

IHC-P: Working dilution: Ready-to-use for 30 min at RT
Staining of formalin-fixed tissues REQUIRES digesting sections with trypsin at 1mg/ml PBS, 10 min at 37oC, followed by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Crude human uterus extract

Cellular localization 

Cytoplasmic

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Myosin

Relevance 

SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SPM201

Isotype 

IgG

Light chain type 

kappa

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.05% Sodium Azide

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Two isoforms: 205kDa (MHC-1) and 200kDa (MHC-2)

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