Anti-N Cadherin antibody [8C11] (ab19348)

I AM LOOKING FOR AN ANTIBODY FOR WESTERN BLOTTING THAT REACTS WITH THE EXTRACELLULAR DOMAIN OF HUMAN N-CADHERIN. COULD YOU LET ME KNOW WHERE THE AB66025 BINDS. THANKYOU

ANSWER:

Thank you for your inquiry.

The immunogen of ab66025 is the full length protein and to our knowledge this clone was not mapped. We therefore do not know where exactly this antibody binds to within the N Cadherin.

According to SwissProt homepage the extracellular domain is aa 160-724.

We have two antibodies that will bind within this sequence and that are tested and guaranteed for WB with human samples:


ab19348
http://www.abcam.com/index.html?datasheet=19348 http://www.abcam.com/index.html?datasheet=19348.

ab95440
http://www.abcam.com/index.html?datasheet=95440 http://www.abcam.com/index.html?datasheet=95440.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

Will ab19348 and ab167 cross-react with E- or N- cadherin, respectively?

ANSWER:

Thank you for your enquiry.

Here is the link to the Clustal W alignment and I will also paste the alignment below. http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20060323-21275762&poll=yes

The immunogen of ab19348 was a bacterial fusion protein corresponding to the extracellular domain of N-cadherin (amino acids 160-724). The immunogen of ab167 was affinity purified 80kD extracellular fragments of E-Cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells (amino acids 155-707). They are both monoclonals so it is difficult to predict what epitope may be recognized as this has not been mapped. The homology is only 46%, so it is likely they do not cross-react and there is no report that they do cross-react, but I cannot guarantee that they do not cross-react.

Here is the alignment so you may make an educated guess:

humanNcad MCRIAGALRTLLPLLLALLQASVEASGEIALCKTGFPEDVYSAVLS-KDVHEGQPLLNVK 59 ecad ----MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVN 56 : : *. *. ** ***.* . * *:.** : *: .:. :.:..*: * .*:

humanNcad FSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAV 119 ecad FEDCTGRQRTAYFSLD-TRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDS-TYRKFSTKV 114 *.:*.*:::. * * : : ***. **:: . *.: : . : :**:** *. * .*:.. *

humanNcad KLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVIPPINLPENSRGPFPQE 179 ecad TLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKN 174 .*. . . :::: . *.: * *:***********. ***.:****::

humanNcad LVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHA 239 ecad LVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHA 234 **:*:*::**: .: **:** *** **.*:***: :* *.**:*****:** : * :**

humanNcad VDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADD-PN 298 ecad VSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVN 294 *. *** **:*::*:*.* * ***:*** ::*::*:* **: *** ** *** **** *

humanNcad ALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQATDMEGNP 358 ecad TYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGE- 353 : *. : * *:** *. *. ******.:** * .*::*****.. ***::**:*::*:

humanNcad TYGLSNTATAVITVTDVNDNPPEFTAMTFYGEVPENRVDIIVANLTVTDKDQPHTPAWNA 418 ecad --GLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEA 411 ***.**********.***** *.. *: *:****..:::::.*.*** * *:****:*

humanNcad VYRISGGDPTGRFAIQTDPNSNDGLVTVVKPIDFETNRMFVLTVAAENQVPLAKGIQHPP 478 ecad VYTILNDD-GGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTT-- 468 ** * ..* *:*.: *:* .***::...* :***::: ::* **. * **: .:

humanNcad QSTATVSVTVIDVNENPYFAPNPKIIRQEEGLHAGTMLTTFTAQDPDRYMQQNIRYTKLS 538 ecad -STATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWR 527 *****:* *:**** * *.* * :. .*.: .* :*::***:** :*:*:* *

humanNcad DPANWLKIDPVNGQITTIAVLDRE-SPNVKNNIYNATFLASDNGIPPMSGTGTLQIYLLD 597 ecad DTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSD 587 *.****:*:* .* *:* * **** :***. *.* ::*:*** * :***** : * *

humanNcad INDNAPQVLPQEAETCETPDPNSINITALDYDIDPNAGPFAFDLPLSPVTIKRNWTITRL 657 ecad VNDNAPIPEPRTIFFCER-NPKPQVINIIDADLPPNTSPFTAELTHG---ASANWTIQYN 643 :***** *: ** :*:. *. :* *: **:.**: :*. . : . ****

humanNcad NGDFAQLNLKIKF-LEAGIYEVPIIITDSGNPPKSNISILRVKVCQCDSNGDCTDVDRIV 716 ecad DPTQESIILKPKMALEVGDYKINLKLMDNQN--KDQVTTLEVSVCDCEGAAGVCRKAQPV 701 : .: ** *: **.* *:: : : *. * *.::: *.*.**:*:. .. : *

humanNcad GAGLGTGAIIAILLCIIILLILVLMFVVWMKRRDKERQAKQLLIDPEDDVRDNILKYDEE 776 ecad EAGLQIPAILGILGGILALLILILLLLLFLRRR---AVVKEPLLPPEDDTRDNVYYYDEE 758 *** **:.** *: ****:*:::::::** .*: *: ****.***: ****

humanNcad GGGEEDQDYDLSQLQQPDTVEPDAIKPVGIRRMDERPIHAEPQYPVRSAAPHPGDIGDFI 836 ecad GGGEEDQDFDLSQLHRGLDARPEVTR-----NDVAPTLMSVPRYLPRPANPD--EIGNFI 811 ********:*****:: ..*:. : : : . .: : *:* *.* *. :**:**

humanNcad NEGLKAADNDPTAPPYDSLLVFDYEGSGSTAGSLSSLNSSSSGGEQDYDYLNDWGPRFKK 896 ecad DENLKAADTDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDKDQDYDYLNEWGNRFKK 871 :*.*****.******************** *.********.*. :*******:** ****

humanNcad LADMYGGGDD- 906 ecad LADMYGGGEDD 882 ********:*

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.