Immunohistochemistry (Frozen sections) abreview for Anti-N Cadherin antibody [EPR1791-4]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic mouse brain coronal cortical section)
Permeabilization
Yes - Triton X 100
Specification
Embryonic mouse brain coronal cortical section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/200

Secondary antibody

Dilution
1/700
Name
Non-Abcam antibody was used: Molecular Probes
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
apical staining in the ventricular zone where N-cadherin forms the main component of adherens junctions. The sections were antigen retrieved using 10mM sodium citrate buffer pH6 in a microwave at 650watts for 10min.
Username

Dr. Bhavin Shah

Verified customer

Submitted Oct 20 2015

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