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Anti-N Cadherin antibody [GC-4] (ab11340)

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Overview

Product name

Anti-N Cadherin antibody [GC-4]
See all N Cadherin products (19) ...

Description

Mouse monoclonal [GC-4] to N Cadherin

Specificity

Monoclonal Anti-N Cadherin/ACAM is specific for a polypeptide (135 kDa), isolated from a freshly prepared extract of rat or chicken cardiac muscle, when assayed by immunoblotting. This antibody reacts with the ACAM molecule in tissues and cells from human, chicken, rat, mouse, and rabbit.

Tested applications

Flow Cyt, ICC/IF, WBmore details

Cross reactivity

Reacts with

Mouse, Chicken, Human

Predicted to work with

Rat, Rabbit

Immunogen

Recombinant full length protein (Chicken heart).

General notes

If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide

Concentration

Concentration information loading...

Purity

Ascites

Primary antibody notes

Monoclonal Anti-N Cadherin/ACAM may be used for the localization of a 135 kDa receptor of the intercellular adherens junction in immunofluorescent microscopy, electron microscopy, and immunoblotting. This antibody can also be used to study the mechanism of adhesion and interaction of cultured cells and cells in tissues. Monoclonal Anti-NCadherin/ACAM stains a large variety of intercellular junctions formed by chicken fibroblastic and epithelial cells in culture. In adult mammals, the antibody stains predominantly heart and lens tissue and also stains a large variety of embryonic tissue. By double labelling, this antibody appears to localize vinculin and actin-rich intercellular contacts and is absent from cellular matrix attachment sites.

Clonality

Monoclonal

Clone number

GC-4

Isotype

IgG1

Research areas

Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins

  • Flow Cytometry - N Cadherin antibody [GC-4] (ab11340)Flow Cytometry - N Cadherin antibody [GC-4] (ab11340) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - N Cadherin antibody [GC-4] (ab11340)Immunocytochemistry/ Immunofluorescence - N Cadherin antibody [GC-4] (ab11340) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: 1/100.
ICC/IF: 1/100.
WB: 1/100. Detects a band of approximately 135 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.

Sequence similarities

Contains 5 cadherin domains.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP19022 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CADH2_HUMAN antibody
  • Cadherin 2 antibody
  • Cadherin 2 N cadherin neuronal antibody
  • Cadherin 2 type 1 antibody
  • Cadherin 2 type 1 N cadherin neuronal antibody
  • cadherin 2 type 1 N-cadherin neuronal antibody
  • Cadherin 2, type 1, N-cadherin (neuronal) antibody
  • Cadherin-2 antibody
  • Cadherin2 antibody
  • Calcium dependent adhesion protein neuronal antibody
  • CD325 antibody
  • CD325 antigen antibody
  • CDH2 antibody
  • CDHN antibody
  • CDw325 antibody
  • CDw325 antigen antibody
  • N cadherin 1 antibody
  • N-cadherin antibody
  • NCAD antibody
  • Neural Cadherin antibody
  • OTTHUMP00000066304 antibody
  • OTTHUMP00000067378 antibody
see all

Database links

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Anti-N Cadherin antibody [GC-4] images:

  Flow Cytometry - N Cadherin antibody [GC-4] (ab11340)

Flow Cytometry - N Cadherin antibody [GC-4] (ab11340)

Flow Cytometry on HeLa cells using ab11340. The antibody was diluted 1:100 and was incubated with the cells for 30 min at 4 °C in 0.2% BSA, 0.1% sodium azide. For more details, please see the abreview.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - N Cadherin antibody [GC-4] (ab11340)

Immunocytochemistry/ Immunofluorescence - N Cadherin antibody [GC-4] (ab11340)

ab11340, at 1/100, staining N cadherin in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. The cells were methanol/acetone (1/1) fixed and blocked with 1% serum and 0.1% BSA prior to incubation with the antibody for 30 minutes. Alexa fluor® 488 chicken polyclonal to mouse IgG, diluted 1/200, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-N Cadherin antibody [GC-4] (ab11340)

This product has been referenced in:

  • McKinney MC & Kulesa PM In vivo calcium dynamics during neural crest cell migration and patterning using GCaMP3. Dev Biol : (2011). ICC/IF; Chicken.Read more (PubMed: 21864525) »
  • Guo Det al. Loss of PI3K? enhances cAMP-dependent MMP remodeling of the myocardial N-cadherin adhesion complexes and extracellular matrix in response to early biomechanical stress. Circ Res 107:1275-89 (2010).Read more (PubMed: 20847309) »

See all 2 publications for this product

Publishing research using ab11340? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"