Anti-N Cadherin antibody (ab12221)

I would like to ask you for help. We need a specific antibody only labeling cardiomyocytes. If possible, the antibody should be labeled already. I have already bought the TroponinI antibody (ab97711) of Abcam which is working quite well. If you could offer another one as desribed, I would be glad. Thank you in advance for your help! Kind regards

ANSWER:

Thank you for contacting us.

There are many proteins that act as markers for Cardiomyocytes; antibodies against these targets can be used for ICC/IF or other required applications.

Main cardiac proteins are as follows;

- alpha actin; alpha sarcomeric Actin antibody [Alpha Sr-1] (ab28052) - N-Cadherin: N Cadherin antibody (ab12221) - alpha actinin; alpha Actinin antibody [0.T.02] (ab18061) - bFGF: FGF basic antibody (ab16828)

I would recommend typing the target name in the search box provided on the Abcam homepage and then selecting the antibodies as per your requirements e.g. monoclonal, polyclonal, rabbit or mouse etc.

The full list of Cardiomyocytes markers can be found by clicking the below link;

http://www.antibodybeyond.com/reviews/cell-markers/cardiomyocyte-marker.htm

Please click on the following link to explore the Abcam Cardiovascular page

http://www.abcam.com/index.html?pageconfig=resource&rid=10694

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

can this antibody be used for immunofluorescence staining in fixed mouse cell lines?

ANSWER:

Thank you for your enquiry.

Yes, the antibody can be used for immunofluorescence staining in fixed mouse cell lines. Please consult our datasheet as well as our Abreviews for this antibody.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

I would like to see the image of stained IHC-P slides using that antibody. The image is not available on line. If you have any reference paper that have already used this antibody I would be pleased to have those references.

ANSWER:

Thank you for your enquiry. I appreciate your patience in this matter.

Following correspondence with the source of this antibody I have obtained an image of this antibody applied using IHC-P. I have uploaded it to the datasheet with immediate effect. It can be viewed by clicking on the link below.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Will this antibody work in dog? Will this antibody cross-react with R cadherin?

ANSWER:

Thank you for your enquiry.

I cannot find the sequence for the gene in dogs. However, if you would like to blast the immunogen sequence, it is listed on the datasheet. If the sequence is highly homologous, it is likely the antibody will work for you.

I blasted the human R-cadherin gene, and found that the R-cadherin protein shares 65% homology with the immunizing peptide. It is therefore possible that the antibody will cross-react.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

I have another customer enquiry regarding ab616. Please refer to her mail:

So far, we have tried using this antibody on frozen sections fixed for 1hour and overnight and at concentrations ranging 1:200; 1:500; 1:1000; 1:2000 and the recommended 1:5000.

We have been able to get weak staining at 1:200 using a fluorescent secondary known as SA Alexa 488. This is the only successfully staining that we have gained thus far. Even using a Tyr amplification kit doesn't seem to improve the stain.

Can you please tell me if you have received any feedback relating to the successful use of this antibody on frozen sections

I have attached some images of our staining, the old antibody staining was not using TSA (amplification) while the new antibody staining used the TSA amplification kit. However, we have also used the new antibody without the TSA kit and staining was unsuccessful.

Any feedback you can provide us with would be greatly appreciated.

I have another customer enquiry regarding ab12221 (N Cadherin antibody (ab12221)). She wants to use Formalin-fixed paraffin-embedded sections and human tissue. Can you recommend what kind of human tissue she should use? Is brain tissue alright to use? Thank you for your time, help and I look forward to your reply.

ANSWER:

Thank you for contacting us for technical support with ab616.

Can you please clarify the type of fixative the customer used? The antibody has been tested in on perfusion fixed (4%PFA) cryostat cut sections or ice cold methanol fixed sections (fixed 10min, 1hr fixation will damage the epitope) so please make sure this is the method used by your customer. Does the customer use triton in the antibody dilution buffer and blocking buffer? We recommend adding 0.3% TritonX100. Can you clarify if the customer used the antibody previously and it worked in their hands without amplification, or have they experienced problems with both batches? Unfortunately we did not receive any files with your e-mail so I cannot look at the images. I hope the above information will help the customer.

I am still trying to find out information regarding the human tissue used for ab12221, my apologies for the delay,