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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Overview
Product name
Anti-N Cadherin antibody
See all N Cadherin products (19) ...
Description
Rabbit polyclonal to N Cadherin
Specificity
This antibody does not react with human E or P Cadherin. It does not inhibit N Cadherin dependent cell-cell contact.
Tested applications
ICC/IF, ICC, WB, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Cow, Human, Zebrafish
Immunogen
Synthetic peptide: RMDERPIHAEPQYPVRSAAP, corresponding to amino acids 808-827 of Human N Cadherin.
RMDERPIHAE PQYPVRSAAP
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - N Cadherin antibody (ab12221)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - N Cadherin antibody (ab12221)
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Immunocytochemistry/ Immunofluorescence - N Cadherin antibody (ab12221)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab12221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100
ICC: Use at an assay dependent concentration. (See Abreview.)
WB: Use a concentration of 5 - 10 µg/ml. Use under non reducing condition. Predicted molecular weight: 99.9 kDa.
IHC-Fr: Use a concentration of 5 - 10 µg/ml.
IHC-P: Use a concentration of 5 - 10 µg/ml.
Target
Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Sequence similarities
Contains 5 cadherin domains.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP19022
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Database links
- Entrez Gene: 414745 Chicken
- Entrez Gene: 281062 Cow
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Entrez Gene: 30291 Zebrafish
- Omim: 114020 Human
- SwissProt: P10288 Chicken
see all
Anti-N Cadherin antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - N Cadherin antibody (ab12221)
Paraffin-embedded sections of 15-day mouse embryo. Antigen retrieval: microwave treatment, 3 minutes x 10 times, 10 mM citrate buffer. Primary antibody: 10 ug/ml ab12221, 1 hour, room temperature. Secondary antibody: Envision plus Colorimetric detection: DAB
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - N Cadherin antibody (ab12221)
ab12221 at 1/200 dilution staining rat heart tissue sections by IHC-P. The tissue was fixed with Histochoice and paraffin sectioned prior to staining. An Alexa-Fluor conjugated donkey anti-rabbit antibody was used as the secondary.
The image depicts stained N-Cadherin (green) and counterstained nuclei (DAPI-blue) overlay (left panel). The DIC (phase) image of the same tissue, superimposed with fluorescence overlay (right panel) confirms the localization of the N-Cadherin at intercalated discs region. Note, the phase contrast image shows typical uninucleated, cylindrical morphology of cardiac myocytes; that are often branched and joined to each other end-to-end (intercalated discs) to form interlacing network.
This image is courtesy of an Abreview submitted by Dr Mal Niladri
Immunocytochemistry/ Immunofluorescence - N Cadherin antibody (ab12221)
ab12221, at 1/100, staining N Cadherin in mouse differentiated embryonic stem cells by immunocytochemistry / immunofluorescence. Cells were methanol/acetone (1/1) fixed and permeabilized in 0.1% triton X prior to blocking in 1% serum and 0.1% BSA for 30 minutes at 21°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary antibody.
This image is courtesy of an Abreview from Sarah Ritson.
Western blot - N Cadherin antibody (ab12221)
All lanes : Anti-N Cadherin antibody (ab12221) at 1/100 dilution
Lane 1 : MDA-MB-231 cells (Negative Control)
Lane 2 : U2OS cells (Positive Control)
Lysates/proteins at 200000 cells per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 99.9 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
This image is courtesy of an anonymous Abreview
Western blot - N Cadherin antibody (ab12221)
All lanes : Anti-N Cadherin antibody (ab12221) at 1/100 dilution
Lane 1 : Whole cell lysate prepared from human glioblastoma cell line U373MG
Lane 2 : Whole cell lysate prepared from human glioblastoma cell line U251
Lysates/proteins at 100 µg per lane.
Secondary
HRP monoclonal anti-rabbit immunoglobulins at 1/7500 dilution
developed using the ECL technique
Predicted band size : 99.9 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 minutes
Distinct bands were found in all tested cell lines with no unspecific bands.
Image courtesy of an anonymous Abreview.
References for Anti-N Cadherin antibody (ab12221)
This product has been referenced in:
- Saghizadeh Met al. Alterations of epithelial stem cell marker patterns in human diabetic corneas and effects of c-met gene therapy. Mol Vis 17:2177-90 (2011). IHC; Human.Read more (PubMed: 21866211) »
- Conant JLet al. Sarcomatoid renal cell carcinoma is an example of epithelial-mesenchymal transition. J Clin Pathol 64:1088-92 (2011).Read more (PubMed: 22003062) »
See all 11 publications for this product
Publishing research using ab12221? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"