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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Overview
Product name
Anti-N Cadherin antibody
See all N Cadherin products (19) ...
Description
Rabbit polyclonal to N Cadherin
Tested applications
IHC-Fr, WB, IHC-P, ICC/IF, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis
Predicted to work with
Chicken, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human N Cadherin.
(Peptide available as ab18620.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Cadherins
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cadherins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/300
WB: Use a concentration of 1 µg/mlDetects a band of approximately 135 kDa (predicted molecular weight: 135 kDa).Can be blocked with N Cadherin peptide (ab18620).
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 5 µg/ml
Flow Cyt: Use a concentration of 10 µg/ml
Target
Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Sequence similarities
Contains 5 cadherin domains.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP19022
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CADH2_HUMAN antibody
- Cadherin 2 antibody
- Cadherin 2 N cadherin neuronal antibody
see all
Database links
- Entrez Gene: 414745 Chicken
- Entrez Gene: 281062 Cow
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P10288 Chicken
- SwissProt: P19022 Human
see all
Anti-N Cadherin antibody images:
Western blot - N Cadherin antibody (ab18203)
All lanes : Anti-N Cadherin antibody (ab18203) at 1 µg/ml
Lane 1 : Rat Brain Lysate
Lane 2 : Mouse Brain Lysate
Lane 3 : Rat Brain Lysate with N Cadherin peptide (ab18620) at 1 µg/ml
Lane 4 : Mouse Brain Lysate with N Cadherin peptide (ab18620) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 135 kDa
Observed band size : 135 kDa
Additional bands at : 50 kDa (possible truncated form).
Immunocytochemistry/ Immunofluorescence - N Cadherin antibody (ab18203)
anti-N cadherin antibody ab18203 stained human embryonic stem cells differentiated into mesoderm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - N Cadherin antibody (ab18203)
IHC image of N Cadherin staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18203, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Flow Cytometry - N Cadherin antibody (ab18203)
ab18203 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7007, a donkey polyclonal to rabbit Ig, was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-N Cadherin antibody (ab18203)
This product has been referenced in:
- Plageman TFet al. Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis. Dev Biol : (2011). IHC-Fr; Mouse.Read more (PubMed: 21726547) »
- Stan Aet al. Essential cooperation of N-cadherin and neuroligin-1 in the transsynaptic control of vesicle accumulation. Proc Natl Acad Sci U S A 107:11116-21 (2010). ICC/IF; Rat.Read more (PubMed: 20534458) »
See all 10 publications for this product
Publishing research using ab18203? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"