Anti-N Cadherin (phospho Y820) antibody (ab76059)

Overview

  • Product nameAnti-N Cadherin (phospho Y820) antibodySee all N Cadherin primary antibodies ...
  • Description
    Rabbit polyclonal to N Cadherin (phospho Y820)
  • Specificityab76059 detects a 130 kDa band corresponding to N cadherin in western blots of serum-starved human endothelial cells treated with pervanadate, but it is not detected in untreated cells.
  • Tested applicationsWB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 820 in human N cadherin.

  • Positive control
    • Human endothelial cells treated with pervanadate (1 mM) for 30 min.
  • General notesDo not aliquot this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 100 kDa.
ELISA 1/2000.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • Sequence similaritiesContains 5 cadherin domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADH2_HUMAN antibody
    • Cadherin 2 antibody
    • Cadherin 2 N cadherin neuronal antibody
    • Cadherin 2 type 1 antibody
    • cadherin 2 type 1 N cadherin neuronal antibody
    • Cadherin 2, type 1, N-cadherin (neuronal) antibody
    • Cadherin-2 antibody
    • Cadherin2 antibody
    • Calcium dependent adhesion protein neuronal antibody
    • CD325 antibody
    • CD325 antigen antibody
    • CDH2 antibody
    • CDHN antibody
    • CDw325 antibody
    • CDw325 antigen antibody
    • N cadherin 1 antibody
    • N-cadherin antibody
    • NCAD antibody
    • Neural Cadherin antibody
    • OTTHUMP00000066304 antibody
    • OTTHUMP00000067378 antibody
    see all

Anti-N Cadherin (phospho Y820) antibody images

  • All lanes : Anti-N Cadherin (phospho Y820) antibody (ab76059) at 1/1000 dilution

    Lane 1 : Human endothelial cells untreated
    Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
    Lane 3 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-820) peptide
    Lane 4 : Human endothelial cells treated with pervanadate (1 mM) for 30 min with phospho-N-cadherin (Tyr-860) peptide


    Predicted band size : 100 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

References for Anti-N Cadherin (phospho Y820) antibody (ab76059)

ab76059 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization Yes - 0.1% triton-x
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
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Verified customer

Submitted Jun 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (differentiated embryonic stem cell)
Specification differentiated embryonic stem cell
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme free
Fixation Formaldehyde
Permeabilization No
Gating Strategy live cells
Username

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Verified customer

Submitted Jul 21 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"