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Western blot - N-PAC antibody (ab69277)
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Immunocytochemistry/ Immunofluorescence - N-PAC antibody (ab69277)
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Product Name
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N-PAC antibody
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Product type
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Primary antibodies
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Description
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Mouse polyclonal to N-PAC
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Immunogen
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Full length human N-PAC protein (AAH64940.1)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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N-PAC transfected 293T cell lysate
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Cellular localization
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Nuclear
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Research areas
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Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
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Relevance
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N-PAC regulates p38 MAP kinase activity by mediating stress activation of p38alpha/MAPK14 and specifically regulating MAPK14 signaling. It indirectly promotes phosphorylation of MAPK14 and activation of ATF2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this N-PAC antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for N-PAC antibody images, references, products related to ab69277 and other tools.
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N-PAC antibody images:
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 Western blot - N-PAC antibody (ab69277)
All lanes : N-PAC antibody (ab69277) at 1/500 dilution
Lane 1 : N-PAC transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
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Immunocytochemistry/ Immunofluorescence - N-PAC antibody (ab69277)
ab69277 at 10µg/ml staining N-PAC in human Hela cells by ICC/IF.
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Search PubMed (MEDLINE) for references to N-PAC
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N-PAC antibody - more information
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Customer reviews (feedback) regarding N-PAC antibody |
Customer FAQs regarding N-PAC antibody |
Protocols for N-PAC antibody |
Price and availability of products related to N-PAC antibody |
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N-PAC antibody - related products:
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N-PAC antibody - other tools:
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Contact Abcam with a Technical Enquiry about N-PAC antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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